Promega this week introduced its GoTaq 2-Step RT-qPCR system, a reagent system for quantitative analysis of RNA.
The first step consists of cDNA synthesis using the GoScript reverse transcription system. This produces full-length cDNAs from rare and abundant targets with up to 5 micrograms of RNA input, the company said.
In the second step, cDNA is quantified using the GoTaq qPCR master mix. Formulated with a hot-start DNA polymerase, GoTaq is optimized for reproducibly sensitive, specific, and highly efficient qPCR using the standard or fast mode of a real-time PCR instrument, Promega said.
The system allows researchers to observe earlier quantification cycle values over broader linear ranges and detect target genes expressed at low levels even with hard-to-amplify samples, the company said.
Sage Science this week launched the Pippin Prep DNA size selection system in the US.
The system automates the collection of DNA fragments for next-generation sequencing library preparation, and replaces manual gel purification techniques that typically require hours, Sage said.
Pippin Prep uses a proprietary electrophoretic approach that extracts narrow size distributions from shared genomic DNA, provides high product recovery, and requires only one to two minutes of labor per sample, the company added.