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Product Watch: Aug 12, 2010

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Promega this week introduced its GoTaq 2-Step RT-qPCR system, a reagent system for quantitative analysis of RNA.

The first step consists of cDNA synthesis using the GoScript reverse transcription system. This produces full-length cDNAs from rare and abundant targets with up to 5 micrograms of RNA input, the company said.

In the second step, cDNA is quantified using the GoTaq qPCR master mix. Formulated with a hot-start DNA polymerase, GoTaq is optimized for reproducibly sensitive, specific, and highly efficient qPCR using the standard or fast mode of a real-time PCR instrument, Promega said.

The system allows researchers to observe earlier quantification cycle values over broader linear ranges and detect target genes expressed at low levels even with hard-to-amplify samples, the company said.


Sage Science this week launched the Pippin Prep DNA size selection system in the US.

The system automates the collection of DNA fragments for next-generation sequencing library preparation, and replaces manual gel purification techniques that typically require hours, Sage said.

Pippin Prep uses a proprietary electrophoretic approach that extracts narrow size distributions from shared genomic DNA, provides high product recovery, and requires only one to two minutes of labor per sample, the company added.

The Scan

WHO Seeks Booster Pause

According to CNN, the World Health Organization is calling for a moratorium on administering SARS-CoV-2 vaccine boosters until more of the world has received initial doses.

For Those Long Legs

With its genome sequence and subsequent RNAi analyses, researchers have examined the genes that give long legs to daddy longlegs, New Scientist says.

September Plans

The New York Times reports that the US Food and Drug Administration is aiming for early September for full approval of the Pfizer-BioNTech SARS-CoV-2 vaccine.

Nucleic Acids Research Papers on Targeting DNA Damage Response, TSMiner, VarSAn

In Nucleic Acids Research this week: genetic changes affecting DNA damage response inhibitor response, "time-series miner" approach, and more.