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Product Watch: May 6, 2010

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Integrated DNA Technologies this week released a double-quenched probe that the company said increases the accuracy and reliability of 5’ nuclease qPCR experiments.

As opposed to traditional probes, which have 20 to 30 bases between the dye and quencher, IDT's probe design positions an internal ZEN quencher nine bases from the 5’ fluorophore. "This shortened distance, particularly when combined with the standard 3’ quencher, significantly decreases background fluorescence and increases sensitivity," the company said.

IDT added that the chemical structure of the ZEN quencher stabilizes duplex formation, which allows it to be used in previously validated sequences.

The company said that the double-quenched probes maintain a consistently low background at 40 base pairs or longer, which results in lower Cq values when compared to traditional probes and leads to an increase in specificity without any loss in sensitivity or quenching efficiency.

The Scan

Push Toward Approval

The Wall Street Journal reports the US Food and Drug Administration is under pressure to grant full approval to SARS-CoV-2 vaccines.

Deer Exposure

About 40 percent of deer in a handful of US states carry antibodies to SARS-CoV-2, according to Nature News.

Millions But Not Enough

NPR reports the US is set to send 110 million SARS-CoV-2 vaccine doses abroad, but that billions are needed.

PNAS Papers on CRISPR-Edited Cancer Models, Multiple Sclerosis Neuroinflammation, Parasitic Wasps

In PNAS this week: gene-editing approach for developing cancer models, role of extracellular proteins in multiple sclerosis, and more.