New Method Uses Input Sample Quantity to Eliminate Need for Control Genes in qPCR | GenomeWeb

NEW YORK (GenomeWeb) — Scientists at the State University of New York's Downstate campus have published a method that could obviate the need to measure control, or housekeeping, genes in quantitative PCR. Using a simple mathematical formula, the technique normalizes efficiency-corrected cycle threshold data to input sample quantity, and is applicable to both standard and high-throughput reverse transcription qPCR.

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