Japanese Biologists Develop REM-PCR to Improve DNA Fusion from Cloning Vectors | GenomeWeb

By Ben Butkus

Japanese biologists have developed a method that allows the fusion of two DNA sequences from separate cloning vectors using a single PCR reaction, according to a recently published research paper.

The method, called restriction enzyme-assisted mega-primer PCR, or REM-PCR, is similar to an existing method called overlap extension PCR, but is less time-consuming and less prone to unwanted errors, according to the scientists.

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