Isothermal Quadruplex Priming Amplification Moves Closer to Point-of-Care MDx Applications | GenomeWeb

NEW YORK (GenomeWeb) — In an effort to design primers that might solve the conundrum of the plateau effect in PCR amplification, researchers at Ohio State University and Ilia State University in the country of Georgia have created a method called quadruplex priming amplification.

The plateau-free method also happens to be isothermal, and is intrinsically fluorescent, making it suitable for use in point-of-care diagnostic assays — an application the OSU group is now working with unnamed entities to develop.

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