Samsung Electronics has been awarded US Patent No. 7,816,315, "Method of isolating a nucleic acid using a material containing an amino group and a carboxyl group and positively charged at a first pH and a solid material for nucleic acid isolation used for the method."
Provides a method of isolating a nucleic acid from a sample; and a solid material for isolating the nucleic acid. The method includes contacting the sample with a bifunctional material containing an amino group and a carboxyl group at a first pH to bind the nucleic acid to the bifunctional material, the bifunctional material being positively charged at the first pH; and releasing the nucleic acid at a second pH, which is higher than the first pH.
Clarity Biosciences has been awarded US Patent No. 7,816,080, "Identifying organisms by detecting intronic nucleic acid or encoded proteins."
Rhonda Honeycutt and Michael McClelland are named as inventors on the patent.
Provides methods for characterizing organisms by identifying the presence, absence, size, or sequence polymorphism of intronic regions. The method involves selecting intronic regions from nuclear or organellar gene sequences that are useful for differentiating between and among taxonomic groupings of organisms. Such intronic regions can be analyzed directly or after amplification in a primer extension reaction. The amplification product is then analyzed by, for example, size fractionation, nucleotide sequencing, or restriction fragment length polymorphism analysis. Intronic regions that contain an open reading frame encoding all or a portion of a protein can be used to generate antibodies to detect the presence or absence of the protein, which indicates the presence or absence of the intronic region. The patent also provides methods of detecting an organism in a sample by detecting the presence or absence of one or more intronic regions using nucleic acid based or immunological based approaches. Kits are provided for practicing the methods of the invention.
Laboratorio de Analisis Dr. Echevarne of Barcelona, Spain, has been awarded US Patent No. 7,816,078, "Method for identifying biological species."
Marcos Riviere is the sole inventor listed on the patent.
Provides a method for identifying species and subspecies in a biological sample through the selective amplification of segments of nucleic acid that code a target region of the cytoplasmatic beta-actin protein, which is present in all the organisms concerned. The method comprises extracting DNA from the sample; amplifying divergent segments of the cytoplasmatic beta-actin gene by PCR or an equivalent technique using primers of regions with high evolutionary conservation between species and subspecies; and identifying the amplified segment by comparing its size in base pairs with a pre-established standard of sizes and/or identifying the amplified segment by DNA sequencing and comparing the resulting sequence with the specific sequence of each species or subspecies present on a computer database.