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IP Watch: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Jul 19, 2011

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The Medical University of South Carolina Foundation for Research Development has been awarded US Patent No. 7,981,616, "Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription."

Michael Mitas, David Cole, William Gillanders, and Kaidi Mikhitarian are named as inventors on the patent.

Discloses truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. The patent also provides forward and reverse primers for RT-PCR, and methods of using the primers.


Esoterix Genetic Laboratories has been awarded US Patent No. 7,981,607, "Method for detecting recombinant event."

Anthony Shuber is named as inventor on the patent.

Describes methods relating to isolating and amplifying chimeric nucleic acid molecules. The methods are useful for detecting chromosome translocation events associated with diseases or conditions, such as cancer.


Roche Molecular Systems has been awarded US Patent No. 7,981,606, "Control for nucleic acid testing."

Thomas Emrich, Gerd Haberhausen, and Martin Moczko are named as inventors on the patent.

Relates to a method for detecting a target biomolecule in a test sample by adding an internal control biomolecule to the test sample. The invention is also related to a method for verifying the determination of a signal indicating the presence of a target biomolecule. The invention further relates to a method for detecting the presence or the absence of a member of a group of target nucleic acids in a sample, and a method for verifying the determination of a signal indicating the presence of a member of a group of target nucleic acids.


Roche Molecular Systems has also been awarded US Patent No. 7,977,108, "Method for detecting a mutation in a repetitive nucleic acid sequence."

Christopher Newhouse, Stephen Will, and Amar Gupta are named as inventors on the patent.

Provides methods and probe nucleic acids for detecting mutant forms of target nucleic acids that comprise repetitive nucleotide sequences. In certain embodiments, for example, the approaches and reagents can be used to detect instability in regions of genomic DNA that include microsatellite markers. The patent also discloses related reaction mixtures, systems, and kits.