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IP Watch: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Feb 22, 2011

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The Mayo Foundation for Medical Education and Research has been awarded US Patent No. 7,893,241, "Articles of manufacture for detection of herpes simplex virus."

Thomas Smith, Arlo Wold, Jim Uhl, and Mark Espy are named as inventors on the patent.

Discloses methods to detect herpes simplex virus in biological samples and to further distinguish between HSV-1 and HSV-2. Also discloses primers and probes for the differential detection of HSV-1 and HSV-2; and articles of manufacture containing such primers and probes for detecting HSV..


The University of Pennsylvania has been awarded US Patent No. 7,892,819, "Mesoscale polynucleotide amplification devices."

Peter Wilding and Larry Kricka are named as inventors on the patent.

Discloses devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction. Provides devices with a substrate microfabricated to include a polynucleotide amplification reaction chamber having at least one cross-sectional dimension of about 0.1 to 1,000 µm. The device also includes at least one port in fluid communication with the reaction chamber, for introducing a sample to the chamber, for venting the chamber when necessary and, optionally, for removing products or waste material from the device. The reaction chamber may be provided with reagents required for amplification of a preselected polynucleotide. The device also may include means for thermally regulating the contents of the reaction chamber to amplify a preselected polynucleotide. Preferably, the reaction chamber is fabricated with a high surface-to-volume ratio to facilitate thermal regulation, the patent's abstract states.


GE Healthcare has been awarded US Patent No. 7,892,797, "Single enzyme system for fast, ultra-long PCR."

Paul Mitsis, Anuradha Sekher, and Gyanendra Kumar are named as inventors on the patent.

Provides methods, formulation, and kits for the synthesis of long nucleic acid fragments. Also provides an improved PCR method for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used to rapidly amplify 10 kb or longer DNA fragments. Also provides a method for extending long complementary DNA strands that uses the aforementioned single enzyme formulation.


EraGen Biosciences has been awarded US Patent No. 7,892,796, "Solid support assay systems and methods utilizing non-standard bases."

James Prudent, David Marshall, Christopher Sherrill, Eric Roesch, Craig Richmond, Christopher Scherrer, Jennifer Greiner, and Jerod Ptacin are named as inventors on the patent.

Describes solid support assays using non-standard bases. More specifically, a capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.


Focus Diagnostics has been awarded US Patent No. 7,892,795, "Methods and compositions for detecting BK virus."

Fan Chen, Lilly Kong, and Jules Chen are named as inventors on the patent.

Provides methods and compositions for rapid, sensitive, and highly specific nucleic acid-based (e.g., DNA based) detection of a BK virus in a sample. In general, the methods involve detecting a target nucleic acid having a target sequence of a conserved region of BK viral genomes. The invention also features compositions, including primers, probes, and kits, for use in the methods of the invention.


Roche Nimblegen has been awarded US Patent No. 7,892,732, "Method of performing PCR amplification on a microarray."

Roland Green and Thomas Albert are named as inventors on the patent.

Provides a method of amplifying target DNA by PCR or multiplex PCR on a microarray using array-immobilized DNA probes synthesized in a common area on the microarray by a maskless array synthesizer. The synthesizer-constructed array-immobilized DNA probes include a universal primer linked to a sequence-specific probe, and optionally a calibrated probe for use in quantifying amplified target DNA.


Stratatech has been awarded US Patent No. 7,888,496, "Kit for species-specific DNA detection."

Lynn Allen-Hoffmann and John Centanni are named as inventors on the patent.

Relates to methods and compositions for identifying species-specific material in pharmaceutical products. In particular, the invention relates to methods for identifying species-specific DNA in a population of a different species or following contact (e.g., growth) with cells of a different species.

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