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IP Watch: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Sep 16, 2010

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InstantLabs Medical Diagnostics has been awarded US Patent No. 7,795,014, "Disposable reactor module and detection system."

Dongqing Li is the sole inventor named on the patent.

Describes a disposable reactor module, a monitoring/optical detection system, and related hardware for chemical reactions, including PCR.


BioMérieux has been awarded US Patent No. 7,794,986, "Method for amplification of RNA sequences."

Birgit Alberta Louisa Maria Deiman and Arnoldina Margaretha Wilhelmina Strijp are named as inventors on the patent.

Relates to a method for amplifying a target RNA sequence. The method includes a first primer that comprises a hybridizing sequence of 7 to 14 nucleotides, which is capable of binding to a first segment of the target RNA sequence, a transcription enhancing sequence, and an anchor that is capable of binding to a second segment of the target RNA sequence. A second primer comprises a hybridizing sequence of 7 to 14 nucleotides, an amplification-enhancing sequence, and an anchor that is capable of binding to a second segment of the first single-stranded cDNA. "The invention further relates to primers for the amplification of target RNA sequences and to a kit comprising one or more of the primers," the patent abstract states.


GHC Technologies has been awarded US Patent No. 7,794,985, "Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins."

Daniel Shoemaker is the sole inventor named on the patent.

Describes a method for detecting the presence of a nucleic acid template in a sample. The method includes the steps of combining the sample in a reaction vessel with a first primer and a second primer having a first section, a second section, and a spacer. The method also includes the steps of extending the first section with additional nucleotides, binding the first primer to the extended first section, extending the first primer with additional nucleotides, and terminating extension of the first primer with the spacer. The first section includes nucleotides that bind with a portion of the nucleic acid template, the second section is spaced apart from the first section and includes nucleotides that do not bind with the nucleic acid template, and the spacer couples the first section to the second section.


Life Genetics Lab has been awarded US Patent No. 7,794,983, "Method for genetic detection using interspersed genetic elements."

Sudhir Sinha are Anthony Carter, are named as inventors on the patent.

According to the patent claims, the patent protects a method for genetic detection that comprises first carrying out PCR on a DNA sample with a primer set to produce amplified DNA products. The primer set includes a first primer for both a filled site containing an interspersed element and an empty site that does not contain the interspersed element, a second primer for the filled site, and a third primer for the empty site. The first primer is complementary to a first flanking genomic sequence in the filled site and the empty site; the second primer is complementary to a sequence comprised of a second flanking genomic sequence, a direct repeat sequence next to the second flanking genomic sequence, and a part of the interspersed element next to the direct repeat sequence in the filled site ; and the third primer is complementary to a sequence comprised of the direct repeat sequence and third flanking genomic sequences on both sides of the direct repeat sequence in the empty site. The method then analyzes the amplified DNA products to detect presence or absence of the interspersed element insertion at the site.


Elitech Holding has been awarded US Patent No. 7,794,945, "Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders."

Joel Hedgpeth, Irina Afonina, Igor Kutyavin, Eugeny Lukhtanov, Evgeniy Belousov, and Rich Meyer Jr. are named as inventors on the patent.

Describes conjugates between a minor groove binding molecule, such as the trimer of CDPI3, and an oligonucleotide that form "unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex." These conjugates can be used as probes and primers in various analytic and diagnostic procedures, including PCR.


Lawrence Livermore National Security has been awarded US Patent No. 7,794,938, "Multiplex detection of agricultural pathogens."

Mary Teresa McBride, Thomas Richard Slezak, and Sharon Lee Messenger are named as inventors on the patent.

Provides kits and methods for detecting seven agricultural pathogens in a sample, including bovine herpes virus, bovine papular stomatitus virus, bovine viral diarrhea, blue tongue virus, swine vesicular disease virus, vesicular exanthema of swine virus, and food tnd mouth disease virus. "Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample," the patent abstract states.


Hologic has been awarded US Patent No. 7,794,935, "Reverse transcriptase compositions for flap-mediated detection of a target nucleic acid sequence."

Joseph Sorge is the sole inventor named on the patent.

Relates to compositions for generating a signal indicative of the presence of a target nucleic acid in a sample. The compositions include a reverse transcriptase, a nuclease, an upstream primer, and a downstream probe.


Piotr Chomczynski has been awarded US Patent No. 7,794,932, "Reagents and methods for isolation of purified RNA."

Describes compositions and methods to isolate intact RNA that is substantially free of DNA. In one embodiment, the sample is treated with phenol at a pH less than 4.0 and purified RNA is recovered from the aqueous phase. In another embodiment, RNA is precipitated from an acidified sample containing a low volume of an organic solvent. Purified RNA obtained by the inventive method may be used in assays where DNA contamination is undesirable, such as PCR.


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