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IP Watch: Kaneka, Hologic, Euclid Diagnostics, University of Missouri Win US Patents

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Kaneka of Osaka, Japan, has been awarded US Patent No. 8,546,131, "Method for detecting nucleic acid, and device or kit."

Shigehiko Miyamoto, Tomohisa Kato, Koji Takahashi, and Jun Tomono are named as inventors.

Discloses a method and a device or kit for simple and precise visual detection of a nucleic acid that has been amplified by a nucleic acid amplification method without the use of special devices. The method comprises contacting a sample with a dye to react with each other, observing a substance produced by the reaction with visible light, and evaluating the presence or absence of a nucleic acid by eye. The device or kit comprises a carrier that holds a dye which can bind to a nucleic acid, a path for passing a sample through the carrier, and an evaluation part for observing a substance produced by the reaction between the sample and the dye with visible light.


Gen-Probe (Hologic) has been awarded US Patent No. 8,546,110, "Method for detecting the presence of nucleic acid in a sample."

Kelly Ammann, Ralph Burns, Ernest Hansberry, Glenn Horner, Cheryl Jakub, John Kling, Donald Nieglos, Robert Schneider, and Robert Smith are named as inventors.

Discloses an automated method for preparing and amplifying a sequence contained in a nucleic acid present in a sample. The nucleic acid is prepared in a receptacle that is part of a unit that includes a plurality of receptacles and holds a removable contact-limiting element for aspirating a fluid component of the sample from the receptacle.


Euclid Diagnostics has been awarded US Patent No. 8,546,078, "Materials and method for assaying for methylation of CpG islands associated with genes in the evaluation of cancer."

Wadiha Freije and Deborah Nusskern are named as inventors.

Provides methods, reagents, and kits for evaluating cancer, such as prostate cancer, in a subject. The patent describes methods of diagnosing and prognosticating cancer, and methods of assessing the efficacy of cancer treatment. The methods include assaying a biological sample for methylation of a CpG island associated with specified genes. The patent provides reagents and kits including primers suitable for amplifying at least a portion of target CpG islands associated with specified genes.


The University of Missouri has been awarded US Patent No. 8,545,769, "Reusable PCR amplification system and method."

Venumadhav Korampally, Shubhra Gangopadhyay, Keshab Gangopadhyay, Sheila Grant, Steven Kleiboeker, Shantanu Bhattacharya, and Yuanfang Gao are named as inventors.

Describes a DNA amplification device utilizing a polydimethylsiloxane (PDMS) and silicon substrate coated with spin-on glass. This PDMS layer is irreversibly bonded to the spin-on glass layer of the silicon substrate using oxygen plasma. The amplification device is an inexpensive, microfluidic device that can be utilized as a portable thermocycler to perform PCR amplification of DNA in the field, the patent's abstract states.

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