Skip to main content

IP Update: Recent Patents Related to PCR, Sample Prep, and Nucleic Acid Amplification: Feb 4, 2010


US Patent No. 7,655,791. DNA amplification and sequencing using DNA molecules generated by random fragmentation. Inventors: Vladimir Makarov, Irina Sleptsova, Emmanuel Kamberov, Eric Bruening. Assignee: Rubicon Genomics.

Describes methods to prepare a DNA molecule or molecules by random fragmentation. In some embodiments, the invention relates to preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3' end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, the methods are used to prepare a library.

Filed under

The Scan

US Supports Patent Waivers

NPR reports that the Biden Administration has announced its support for waiving intellectual property protections for SARS-CoV-2 vaccines.

Vaccines Versus Variants

Two studies find the Pfizer-BioNTech SARS-CoV-2 vaccine to be effective against viral variants, and Moderna reports on booster shots to combat variants.

CRISPR for What Ails You

The Wall Street Journal writes that CRISPR-based therapies could someday be used to treat common conditions like heart attacks.

Nature Papers Review Integration of Single-Cell Assay Data, Present Approach to Detect Rare Variants

In Nature this week: review of ways to integrate data from single-cell assays, and more.