US Patent No. 7,655,791. DNA amplification and sequencing using DNA molecules generated by random fragmentation. Inventors: Vladimir Makarov, Irina Sleptsova, Emmanuel Kamberov, Eric Bruening. Assignee: Rubicon Genomics.
Describes methods to prepare a DNA molecule or molecules by random fragmentation. In some embodiments, the invention relates to preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3' end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, the methods are used to prepare a library.