US Patent No. 7,674,582. Methods for amplification monitoring and gene expression profiling involving real-time amplification reaction sampling.
Inventor: Vladimir Slepnev
Relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest, particularly in real time. The patent provides methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more sequences from two or more samples. The monitoring methods permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.