A team of Broad Institute researchers has developed a panel of qPCR assays designed to identify the primary causes of PCR amplification bias in Illumina sequencing libraries, and built an optimized protocol that can help reduce such bias, according to a recent paper.

The scientists said they expect the new protocol to amplify sequencing libraries more evenly than the standard Illumina protocol, and to minimize bias introduced by factors such as choice of thermocycler and PCR amplification enzyme, and temperature ramp rate.

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