At Threshold of Detection, False-Positive Droplets a Nuisance in ddPCR

Feb 13, 2014

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This article has been updated from a previous version to include comments from Bio-Rad.

Quantifying HIV levels in patients on antiviral therapy demands assays able to function well in the lowest ranges. A recent study in PLOS One has shown seminested qPCR to be more reliable for detecting ultra-low levels of cell-associated HIV RNA than droplet digital PCR, in part due to fluorescence in no-template control droplets.

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