Study Claims PCR from Primers Annealed in Parallel Orientation

Feb 06, 2015

|

Premium

NEW YORK (GenomeWeb) – Researchers in India claim to have accomplished replication of DNA in a parallel orientation, challenging the dogma that PCR can only be initiated when primers are aligned in an anti-parallel way

In a study published ahead of peer review in the online journal F1000 Research, the scientists described how they accomplished parallel DNA PCR, or PD-PCR, using single-stranded template DNA and Taq Polymerase.

Get the full story with
GenomeWeb Premium

Only $95 for the
first 90 days*

GenomeWeb Premium gives you:
✔ Full site access
✔ Interest-based email alerts
✔ Access to archives

Never miss another important industry story.

Try GenomeWeb Premium now.

You may already have institutional access!

Check if I qualify.

Already a GenomeWeb or 360Dx Premium member?
Login Now.

*Before your trial expires, we’ll put together a custom quote with your long-term premium options.

Not ready for premium?

Register for Free Content
You can still register for access to our free content.

Breaking News

The Scan

Genome of an Oak

A new study catalogues the genome and evolutionary history of the oak family, UPI reports.

'Mostly Chihuahua, or Mostly Rat Terrier'

Dog DNA testing is a growing market, but there's still a lot of uncertainty about the accuracy of the results, the Boston Globe says.

Amelia, Is That You?

A University of South Florida researcher is testing bone fragments to determine if they belong to Amelia Earhart.

Cell Papers Explore Koala Genome Retrovirus Responses, Liver Cancer Proteomics, More

In Cell this week: antisense Piwi-interacting RNA responses to endogenous retroviruses, proteomic patterns in hepatocellular carcinoma, and more.

Featured White Papers

Efficient High-Throughput SNP Discovery and Genotyping using Normalized Genotyping-by-Sequencing

SeqSNP Targeted GBS as Alternative for Array Genotyping in Routine Breeding Programs

SeqSNP: A Massively Parallel Marker Screening Approach – High-Speed and High-Throughput Genomic Selection for Plants and Livestock

Sample Normalization with RNAGEM

Oct

23

Featured Webinar

Single-Cell Methylation Profiling of Pediatric Tumors via a Unique NGS-based Approach

This webinar will illustrate how single-cell methylation sequencing can be applied to gain significant insight into epigenetic heterogeneity in disease states, advancing cancer research discoveries.

Oct

24

Featured Webinar

Designing, Decoding & Delivering on Genetic Testing for Inherited Disease at Versiti

This webinar will tell the story of Versiti’s journey in transforming genetic testing from a manual to a digitized process. It will include detail on how the organization succeeded, pain points along the way, a novel approach to variant assessment, and future plans for the program.

Dec

02

Featured Webinar

Comprehensive Profiling of Myeloid Malignancies with Reliable Detection of FLT3 and CEBPA

This webinar will discuss how Moffitt Cancer Center has implemented a new capture-based application to accurately assess myeloid malignancies by detecting complex variants in challenging genes in a single experiment.  

Menu