Close Menu
Home » SickKids Researchers Publish Pre-PCR Method for SNPs in GC-rich Regions

SickKids Researchers Publish Pre-PCR Method for SNPs in GC-rich Regions

Dec 22, 2014
|
Madeleine Johnson
Premium

NEW YORK (GenomeWeb) – The human genome has an estimated 300,000 known SNPs that lie in regions of high GC content, according to researchers at The Hospital for Sick Children and the University of Toronto. Probe-based PCR assays often fail in these regions, but new research from the SickKids-Toronto team has now shown that adding a pre-amplification step to TaqMan SNP genotyping protocols allows better discrimination.

Get the full story with
GenomeWeb Premium

Only $95 for the
first 90 days*

GenomeWeb Premium gives you:
✔ Full site access
✔ Interest-based email alerts
✔ Access to archives

Never miss another important industry story.

Try GenomeWeb Premium now.

You may already have institutional access!

Check if I qualify.

Already a GenomeWeb or 360Dx Premium member?
Login Now.

*Before your trial expires, we’ll put together a custom quote with your long-term premium options.

Not ready for premium?

Register for Free Content
You can still register for access to our free content.

The Scan

Metabolite Trail

The Economist reports that it is increasingly easier to analyze the metabolites people give off, potentially revealing personal information about them.

Just a Few Changes...

A controversial paper on the gender gap in science has been corrected, according to BuzzFeed News.

Not Meeting Standards

The Los Angeles Times reports that only a third of California students meet the state's new science standards.

Science Studies Examine Archaic Admixture in West Africans, Variant Linked to Cannabis Dependence Risk

In Science this week: evidence of interbreeding between the ancestors of West Africans and an unknown archaic human, and more.

Feb
25
Featured Webinar

Long-Read Sequencing of Human Transcription Provides Insights into Metastatic Cancer

Sponsored by
Loop Genomics

This webinar will discuss a study that used long-read transcriptome sequencing to explore the distribution of isoforms in colon cancer samples and their metastasis counterparts. 

Feb
26
Featured Webinar

Transformation of the Lab to Fight Genetic Illnesses in the Amish and Mennonite Populations

Sponsored by
Autogen

This webinar will explain how the Clinic for Special Children in Strasburg, Pennsylvania, has transformed its DNA workflows to improve the diagnosis and treatment of genetic illnesses that are prevalent in the pediatric population of its community.

Feb
27
Featured Webinar

Digital PCR Method Development for Accurate Quantification of Copy Number Variations

Sponsored by
Stilla

Since the publication of the “The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments” (dMIQE) in 2013, there has been a large expansion of the applications of dPCR such as single nucleotide variations (SNVs) and copy number variations (CNVs) measurements associated with disease diagnostics.

Mar
18
Featured Webinar

Comparison of Genome-Wide versus Targeted cfDNA Testing for First-Line Prenatal Screening: Real-World Data

Sponsored by
Roche

This webinar will discuss data from a recent real-world comparison study evaluating performance of two cell-free DNA methodologies as first-line prenatal screens.

What's Popular?

In PCR

  1. BGI Distributes More Than 230,000 Coronavirus Test Kits

  2. Diagnostics Firms Rush to Develop Rapid Point-of-Care Tests for Novel Coronavirus

    Premium

  3. Qiagen Readying Updated Respiratory Panel for Emergency 2019-nCoV Testing

  4. POC Molecular Flu Tests Finding Resistance in Pediatrics Despite High Accuracy

    Premium

  5. Coronavirus Test Kits Encounter QC Hiccup in Some States, CDC Reports

Sponsorships

Privacy Policy.  Terms & Conditions.  .  Copyright © 2020 GenomeWeb, a business unit of Crain Communications.  All Rights Reserved.