Researchers Describe 'Digital PLA' Method to Quantify mRNA, Protein in Single Cells

Mar 28, 2016

|

Premium

NEW YORK (GenomeWeb) – Even clonal populations of cells can be heterogeneous relative to the central dogma of biology, with "noise" leading to variability in mRNA and protein copy numbers.

However, determining how many proteins are produced per mRNA in a given cell has long been a challenge. Now scientists at ETH Zurich, University of Bern, and the University of Chicago have developed a new method that uses digital PCR (dPCR) to quantify both mRNA and the DNA products of a proximity ligation assay (PLA) for proteins.

Get the full story with
GenomeWeb Premium

Only $95 for the
first 90 days*

GenomeWeb Premium gives you:
✔ Full site access
✔ Interest-based email alerts
✔ Access to archives

Never miss another important industry story.

Try GenomeWeb Premium now.

You may already have institutional access!

Check if I qualify.

Already a GenomeWeb or 360Dx Premium member?
Login Now.

*Before your trial expires, we’ll put together a custom quote with your long-term premium options.

Not ready for premium?

Register for Free Content
You can still register for access to our free content.

Breaking News

The Scan

Asked to Give DNA Back

A South African university has told the Wellcome Sanger Institute to return DNA samples it has from indigenous African communities, The Times reports.

CCR5 Gene Mortality Paper Retracted

The University of California, Berkeley's Rasmus Nielsen and Xinzhu Wei have retracted their CCR5 gene paper due to a technical artifact.

Catch It Before Being Hospitalized

University of Virginia researchers are exploring a genetic risk test to gauge type 1 diabetes risk, NPR reports.

PNAS Studies Look at Lung Cancer Models, Height in Ancient Europeans, More

In PNAS this week: researchers compare two high-grade neuroendocrine lung cancers, height among ancient Europeans, and more.

Featured White Papers

Efficient High-Throughput SNP Discovery and Genotyping using Normalized Genotyping-by-Sequencing

SeqSNP Targeted GBS as Alternative for Array Genotyping in Routine Breeding Programs

SeqSNP: A Massively Parallel Marker Screening Approach – High-Speed and High-Throughput Genomic Selection for Plants and Livestock

Sample Normalization with RNAGEM

Oct

23

Featured Webinar

Single-Cell Methylation Profiling of Pediatric Tumors via a Unique NGS-based Approach

This webinar will illustrate how single-cell methylation sequencing can be applied to gain significant insight into epigenetic heterogeneity in disease states, advancing cancer research discoveries.

Oct

24

Featured Webinar

Designing, Decoding & Delivering on Genetic Testing for Inherited Disease at Versiti

This webinar will tell the story of Versiti’s journey in transforming genetic testing from a manual to a digitized process. It will include detail on how the organization succeeded, pain points along the way, a novel approach to variant assessment, and future plans for the program.

Dec

02

Featured Webinar

Comprehensive Profiling of Myeloid Malignancies with Reliable Detection of FLT3 and CEBPA

This webinar will discuss how Moffitt Cancer Center has implemented a new capture-based application to accurately assess myeloid malignancies by detecting complex variants in challenging genes in a single experiment.  

Menu