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Product Watch: Roche LightCycler Software; Sigma Life Science OligoEvaluator


Roche this week launched new software updates, including advanced high-resolution melting analysis and qualitative detection modules, for the LightCycler 96 system.

The new LightCycler 96 module for high-resolution melting analysis will be particularly valuable in human genetic disease research applications, Roche said. It enables easy and fast detection of known and unknown gene mutations, including SNPs, deletions, and methylated regions. Meantime, the dedicated qualitative detection module for LightCycler 96 enables direct detection and validation of a target gene's presence or absence. This module also employs a novel control concept and is designed mainly for customers doing research in the field of infectious diseases, Roche said.

The company also introduced new support features for the LightCycler Nano, including advanced gene expression analysis mediated by the import of external standard curves, and an enhanced analysis method for high-resolution melting analysis.

Sigma-Aldrich said today that its Sigma Life Science business has launched OligoEvaluator, a free online tool based on Premier Biosoft's algorithms that lets scientists analyze the physical properties of oligonucleotide sequences ahead of PCR and other techniques.

OligoEvaluator calculates useful and common oligonucleotide properties, such as molecular weight and melting temperature, as well as oligonucleotide resuspension and dilution volumes. Sequences can also be BLAST searched to check for off-target hybridization, Sigma said.

The tool can be accessed here.

The Scan

Enzyme Involved in Lipid Metabolism Linked to Mutational Signatures

In Nature Genetics, a Wellcome Sanger Institute-led team found that APOBEC1 may contribute to the development of the SBS2 and SBS13 mutational signatures in the small intestine.

Family Genetic Risk Score Linked to Diagnostic Trajectory in Psychiatric Disorders

Researchers in JAMA Psychiatry find ties between high or low family genetic risk scores and diagnostic stability or change in four major psychiatric disorders over time.

Study Questions Existence of Fetal Microbiome

A study appearing in Nature this week suggests that the reported fetal microbiome might be the result of sample contamination.

Fruit Fly Study Explores Gut Microbiome Effects on Circadian Rhythm

With gut microbiome and gene expression experiments, researchers in PNAS see signs that the microbiome contributes to circadian rhythm synchronicity and stability in fruit flies.