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Product Watch: IDT's PrimeTime qPCR Probes; LGC's Oktopure NA Extraction System

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Integrated DNA Technologies has launched PrimeTime Eco Probes and PrimeTime Mini LNA Probes as part of its PrimeTime qPCR products portfolio.

Developed to support customers seeking a cost-effective option at the mid-scale range, the Eco Probe has a normalized yield of 2.5 nanomoles, sufficient for approximately 500 reactions, IDT said. If ordered with the option of an internal ZEN quencher, the double-quenched PrimeTime Eco Probe will generate less background noise, increased endpoint signal, and significantly greater qPCR sensitivity and precision when compared to traditional single-quenched probes. The Eco Probe is available with a FAM/ZEN/IA Black FQ dye/quencher combination, IDT said.

Meantime, PrimeTime Mini LNA Probes enable customers to carry out as few as 100 PCR genotyping reactions, allowing preliminary experiments to be performed at a low initial cost in order to test and consequently optimize LNA probe design.

IDT provides guidelines for LNA probe design online, and can provide full design support, if required, although fees may apply, the company said. The Mini LNA Probes complement the existing 250-nanomole PrimeTime LNA Probes, which provide 8,000 reactions and are ideal for large-scale screening, IDT said.


LGC Genomics recently launched the Oktopure automated nucleic acid extraction platform.

Oktopure combines higher throughput with high-quality DNA extraction and is capable of processing eight 96-well microtiter plates in approximately 1.5 hours, the company said. Oktopure is compatible with all existing laboratory systems, including LGC's SNPline for high-throughput genotyping with KASP, a homogeneous, fluorescence-based PCR assay that enables accurate bi-allelic discrimination of known SNPs and indels, LGC said.

In addition, the ability to use Oktopure in combination with a tip wash station enables the reuse of tips in 40 consecutive extractions, thereby reducing customers' consumable costs.

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