GE Healthcare has launched its Illustra Ready-To-Go GenomiPhi kits, providing researchers with a pre-dispensed, room temperature stable formulation for whole-genome amplification.
The new kits simplify the workflow for obtaining large amounts of high-quality DNA from small genomic DNA samples, and deliver improved yields over current GenomiPhi kits, GE Healthcare said.
The kit is based on Phi29 DNA polymerase, which offers high fidelity and processivity. While previous GenomiPhi kits contain liquid enzyme formulations that require storage at -80° C, the new kits use GE's Ready-To-Go stabilization technology, allowing the kits to be stored in a solid format for months without refrigeration.
The new kits are available in two formats: Ready-To-Go GenomiPhi V3, which improves upon current V2 kits with more than double the DNA yield; and Ready-To-Go GenomiPhi HY, which is designed for high-yield requirements, achieving 40 µg to 60 µg of DNA yield from just 10 ng of starting material.
Sigma Life Science has launched an enhanced version of OligoArchitect, the company's free online tool for automated design of primers and probes for qRT-PCR.
OligoArchitect is powered by the Beacon Designer platform from Premier Biosoft. In addition to the original dual-labeled probe (hydrolysis probes, used in the 5' nuclease assay) and SYBR Green I primer design capabilities found in previous versions, the new OligoArchitect includes design modules for Molecular Beacons, Scorpions probes, and LightCycler probes.