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Product Watch: Mar 9, 2010


Fluidigm has released 5-kilobase and 10-kilobase long-range PCR protocols for its Access Array system, allowing users to amplify up to 48 samples per array for use in targeted resequencing.

Fluidigm said that the new protocols allow users to enrich up to 240 kb per sample and 11.5 megabases per array, or 480 kb and 23 Mb per array, respectively, from 48 samples at a time.

The Access Array system uses as little as two microliters of PCR master mix per 48 amplicons, and the entire sample-prep workflow requires three pipette steps per 48 amplicons, according to the company. This enables the enrichment of up to 23 Mb per array in as little as four hours and for less than $7 per sample, Fluidigm said.

Access Array is compatible with several next-generation sequencing platforms, including the Roche 454 FLX, Illumina Genome Analyzer II, and Life Technologies SOLiD 4.

Epigentek has launched its BisulFlash DNA Modification Kit, which can convert unmethylated cytosine into uracil in around 30 minutes, the company said.

The converted DNA eluate can be obtained for use in mutagenically separated PCR, real-time MS-PCR, methylation microarray, and methylation sequencing and pyrosequencing applications, the company said.

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