Skip to main content
Premium Trial:

Request an Annual Quote

NuGen, Roche's 454 Team to Provide Sample Prep for Low-Abundance RNA Sequencing


This article was originally published on April 5.

Roche's 454 Life Sciences and NuGen Technologies are collaborating to offer sample preparation and high-throughput sequencing of small and degraded RNA samples, the companies said today.

Under the agreement, the companies will co-promote NuGen's Ovation RNA-Seq system with 454 Life Sciences' GS FLX and GS Junior systems to enable whole-transcriptome profiling of low-abundance samples, the companies said.

More specifically, the companies have developed a method that uses NuGen's proprietary isothermal linear amplification products and the GS FLX Titanium cDNA rapid library preparation protocol for the GS Junior and GS FLX.

The companies said that their collaboration aims to help researchers maximize the use of low-abundance samples, since typical cDNA protocols often require as much as 20 µg of input material.

Combining NuGen's sample prep products with 454's sequencing systems will allow researchers to perform whole-transcriptome profiling on samples with as little as 500 pg of total RNA, NuGen CEO Elizabeth Hutt said in a statement.

"Being able to collaborate with a leading NGS platform provider such as Roche enables customers to leverage NuGen's linear amplification technology while obtaining long and highly accurate sequence reads," Nutt said.

Todd Arnold, vice president of development at 454, added that the combined product offering meets the need of researchers working with precious or irreplaceable samples while allowing them to take advantage of the 454 sequencers' long read lengths, "which are critical for both de novo transcriptome assembly and comprehensive identification of splice variants and fusion transcripts."

The Scan

Two J&J Doses

Johnson & Johnson says two doses of its SARS-CoV-2 vaccine provides increased protection against symptomatic COVID-19, CNN reports.

Pfizer-BioNTech Vaccine Response in Kids

The Pfizer-BioNTech SARS-CoV-2 vaccine in a lower-dose format appears to generate an immune response among children, according to the Washington Post.

Chicken Changes to Prevent Disease

The Guardian writes that researchers are looking at gene editing chickens to help prevent future pandemics.

PNAS Papers on Siberian Dog Ancestry, Insect Reproduction, Hippocampal Neurogenesis

In PNAS this week: ancestry and admixture among Siberian dogs, hormone role in fruit fly reproduction, and more.