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IP Watch: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Oct 6, 2010


Third Wave Technologies (now Hologic) has been awarded US Patent No. 7,807,806, "RNA detection assays."

Hatim Allawi, Brad Argue, Christian Bartholomay, LuAnne Chehak, Michelle Curtis, Peggy Eis, Jeff Hall, Hon Ip, Lin Ji, Michael Kaiser, Robert Kwiatkowski, Andrew Lukowiak, Victor Lyamichev, Natalie Lyamicheva, Wupo Ma, Bruce Neri, Sarah Olson, Marilyn Olson-Munoz, James Schaefer, Zbigniev Skrzypczynski, Tsetska Takova, Lisa Thompson, and Kevin Vedvik are named as inventors on the patent.

Provides novel cleavage agents and polymerases for cleaving and modifying nucleic acid. The cleavage agents and polymerases find use, for example, for detecting and characterizing nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

US Patent No. 7,807,802, "Polynucleotides for the amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae," has been awarded to an unnamed entity.

Edward Pabich, Ronald Marshall, and Hong Yu are named as inventors on the patent. Abbott Laboratories is the corresponding entity on the patent application.

Discloses polynucleotides useful for detecting Neisseria gonorrhoeae in a test sample; kits; a nucleic acid amplification method; and a nucleic acid detection method.

BioMérieux has been awarded US Patent No. 7,807,449, "Nucleic acid amplification reaction station for disposable test devices."

Bryan Kluttz, Geoff McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catazariti, Louis Graziano, Bruno Colin, Cecile Paris, and Jacque Dachaud are named as inventors on the patent.

Discloses an instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing or in fluid communication with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as reverse transcriptase). The instrument includes a support structure receiving the test device. A temperature-control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

Cornell University has been awarded US Patent No. 7,807,431, "Detection of nucleic acid differences using combined endonuclease cleavage and ligation reactions."

Francis Barany, Weiguo Cao, Jianmin Huang, and Jing Lu are named as inventors on the patent.

Discloses a method for detecting DNA sequence differences including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions. The method includes preparing labeled heteroduplex PCR fragments containing base mismatches. Endonuclease cleaves the heteroduplex PCR fragments both at the position containing the variation (one or more mismatched bases) and, to a lesser extent, at non-variant (perfectly matched) positions. Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background. This is then followed by a detection step in which the reaction products are detected and the position of the sequence variations are determined, the patent's abstract states.

Life Technologies has been awarded US Patent No. 7,807,376, "Modified oligonucleotides and applications thereof."

Bashar Mullah and Zhaochun Ma are named as inventors on the patent.

Discloses, among other things, primers containing certain modified nucleobases in the 3' terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof.

Samsung has been awarded US Patent No. 7,807,360, "Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber."

Young-rok Kim, Jun-hong Min, In-ho Lee, Young-sun Lee, Chang-eun Yoo, and Ki-woong Han are named as inventors on the patent.

Discloses a method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber. The method includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding it on the interior surface of the micro chamber; and performing PCR by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber, thereby reducing consumables, time, and labor for treatment and analysis; improving detection sensitivity; and significantly reducing risk of sample cross-contamination without sample loss by eliminating transporting of the sample. The patent also provides a complete automated system for concentration and amplification of nucleic acid.

Enzo Life Sciences has been awarded US Patent No. 7,807,352, "Process for producing two or more copies of nucleic acids in a library, and process for detecting or quantifying more than one nucleic acid in a library."

Elazar Rabbani, Jannis Stavrianopoulos, James Donegan, and Jack Coleman are named as inventors on the patent.

Provides novel compositions and processes for analyte detection, quantification, and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities, and the like are employed to detect and, if necessary or desirable, to quantify analytes. The invention also provides compositions and processes related to the amplification of target analytes.

National Taiwan University has been awarded US Patent No. 7,807,347, "Immuno-PCR method for detecting nasopharyngeal carcinoma markers and kit thereof."

Hsiang-Yin Lu, Tzu-Wei Wang, Feng-Huei Lin, and Pei-Jen Lou are named as inventors on the patent.

Relates to an immuno-PCR method for detecting nasopharyngeal carcinoma (NPC), especially markers of early stage NPC, and kit thereof. The invention comprises providing a substrate on which protein markers are immobilized; applying a patient's specimen to the substrate; adding a solution that has biotinylated anti-human IgA secondary antibody; incubating the solution; adding a solution with a linker and biotinylated target DNA; conducting a polymerase chain reaction; and detecting the target DNA fragments via electrophoresis.

The Scan

Researchers Develop Polygenic Risk Scores for Dozens of Disease-Related Exposures

With genetic data from two large population cohorts and summary statistics from prior genome-wide association studies, researchers came up with 27 exposure polygenic risk scores in the American Journal of Human Genetics.

US Survey Data Suggests Ancestry Testing Leads Way in Awareness, Use of Genetic Testing Awareness

Although roughly three-quarters of surveyed individuals in a Genetics in Medicine study reported awareness of genetic testing, use of such tests was lower and varied with income, ancestry, and disease history.

Coral Genome Leads to Alternative Amino Acid Pathway Found in Other Non-Model Animals

An alternative cysteine biosynthesis pathway unearthed in the Acropora loripes genome subsequently turned up in sequences from non-mammalian, -nematode, or -arthropod animals, researchers report in Science Advances.

Mosquitos Genetically Modified to Prevent Malaria Spread

A gene drive approach could be used to render mosquitos unable to spread malaria, researchers report in Science Advances.