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IP Watch: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Aug 10, 2011

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DxNA of St. George, Utah, has been awarded US Patent No. 7,993,910, "Methods and apparatus for amplification of DNA using sonic energy."

William Bickmore and Danvern Ray Roberts are named as inventors on the patent.

Discloses an apparatus and methods for amplifying DNA that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double-stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application and cessation of application of sonic energy results in DNA amplification.


Genoid of Budapest, Hungary, has been awarded US Patent No. 7,993,881, "Method for detecting pathogens using molecular beacons."

Csaba Jeney and Tibor Takacs are named as inventors on the patent.

Discloses a method for detecting pathogens, particularly organisms associated with sexually transmitted diseases, especially human papillomavirus genotypes. The method involves the use of real-time PCR using specially designed probes. The patent also describes probes, kits for carrying out the method, and methods for designing primers suitable for use in the method.


Stanford University has been awarded US Patent No. 7,993,880, "Precircle probe nucleic acid amplification methods."

Thomas Willis, Paul Hardenbol, Maneesh Jain, Viktor Stolc, Mostafa Ronaghi, and Ronald Davis are named as inventors on the patent.

Discloses methods of multiplexing nucleic acid reactions, including amplification, detection, and genotyping. The invention relies on the use of precircle probes that are circularized in the presence of the corresponding target nucleic acids; cleaved; and then amplified.


Life Technologies has been awarded US Patent No. 7,993,842, "Directed enrichment of genomic DNA for high-throughput sequencing."

Kevin McKernan, Alan Blanchard, and Douglas Smith are named as inventors on the patent.

Discloses microarrays of oligonucleotide primer pairs and, in particular, microarrays of primers that comprise at least one cleavable linkage. Also provides methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured primer pairs, such as for amplification of a target polynucleotide sequence. In addition, the patent also provides methods of using a microarray to isolate, purify, and/or amplify a target polynucleotide.


Eppendorf has been awarded US Patent No. 7,993,840, "Detection and/or quantification method of target molecules on a solid support."

Isabelle Alexandre, Heinz Koehn, Jose Remacle, and Sven De Roeck are named as inventors on the patent.

Relates to a method and device for detecting and/or quantifying one or multiple target molecules present in a solution by quantifying online their binding on specific capture molecules immobilized at different locations of a surface of an optically transparent solid support without substantial detection of target molecules present in solution. The invention allows the performance and simultaneous detection of multiple target assays. More particularly, the invention comprises detecting in real time the hybridization between capture DNA molecules present on a microarray and target polynucleotides present in solution. The invention also relates to real-time PCR of multiple targets on a microarray.


General Electric has been awarded US Patent No. 7,993,839, "Methods and kits for reducing non-specific nucleic acid amplification."

John Nelson, Robert Duthie, Sonali Shah, and Clifford Smith are named as inventors on the patent.

Provides methods and kits for efficient amplification of nucleic acids. The methods comprise in vitro amplification of a nucleic acid template employing partially constrained primers having a terminal mismatch primer-dimer structure. The methods also comprise in vitro amplification of a nucleic acid template employing partially constrained primers having nucleotide analogues. The methods enhance efficiency of nucleic acid amplification reactions by reducing non-specific amplification.


Asuragen has been awarded US Patent No. 7,993,831, "Methods of normalization in microRNA detection assays."

Gary Latham and Heidi Peltier are named as inventors on the patent.

Describes methods of quantifying a target miRNA in a biological sample by measuring the amounts of a target miRNA and at least one reference oncomir in a reaction volume, and normalizing the amount of target miRNA to the amount of one or more reference oncomirs.


Siemens has been awarded US Patent No. 7,993,828, "PCR process and arrangement for DNA amplification using dry reagents."

Walter Gumbrecht, Peter Paulicka, and Manfred Stanzel are named as inventors on the patent.

Discloses a PCR process for DNA amplification with thermocycling of corresponding reagents. The process achieves total integration of all substances and process in a closed, single-use unit or cartridge in which the reagents are stored in a storage-stable form at room temperature. The water-soluble reagents are covered by a water-insoluble medium; then, the DNA to be amplified is supplied and the water-insoluble medium is eliminated so that the water-soluble reagents are dissolved and PCR can start. The patent also describes a test unit designed as a single-use product or cartridge having at least one micro-channel or micro-cavity for receiving a PCR reagent. The PCR reagents are in a mixture that can be dried at a negligible vapor pressure and which forms a storage-stable substance at room temperature that adheres to the walls of the micro-channel or micro-cavity and forms a thin film covered by an insoluble medium, according to the patent's abstract.

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