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IP Watch: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Sep 8, 2011

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Olink has been awarded US Patent No. 8,013,134, "Kit for proximity probing with multivalent proximity probes."

Simon Fredriksson is the sole inventor named on the patent.

The patent describes assays for detection and/or quantification of one or more analytes in solution using multivalent proximity probes. The proximity probes each comprise several binding moieties, such as antibodies, and associated nucleic acids. When the binding moieties have bound to their analytes, the nucleic acids on opposite proximity probes interact with each other and a signal is generated based on this interaction that can be detected and quantified by an amplification method such as PCR.


US Patent No. 8,012,719, "Method for the quantitative detection of nucleic acids," has been awarded to inventors Paolo Lusso, Mauro Malnati, and Giulia Cassina.

Covers a method for the quantitative detection of HHV-6 subtypes A and B based on the use of a calibrator, "suitable primers and probes," and a nucleic acid polymerase with 5'-3' nuclease activity, according to the patent abstract.


454 Life Sciences (now part of Roche) has been awarded US Patent No. 8,012,690, "Bead emulsion nucleic acid amplification."

Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, and Michael P. Weiner are named as inventors on the patent.

Protects methods for nucleic acid amplification in which nucleic acid templates, beads, and an amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. The patent also describes kits and apparatuses for performing the methods of the invention.


Applied Biosystems (now Life Technologies) has been awarded US Patent No. 8,012,691, "Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors."

Jaiprakash G. Shewale, Manohar Furtado, Pius Brzoska, Maura Barbisin, Rixun Fang, Michael Malicdem, and Cristin O'Shea are named as inventors on the patent.

Describes a method for "simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction," according to the patent abstract. The multiplex quantification method provides a ratio of human nuclear and male DNA present in a biological sample, which is "useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping."


Life Technologies has been awarded US Patent No. 8,012,688, "Gene expression profiling from FFPE samples."

Mark G. Erlander and Ranelle C. Salunga are named as inventors on the patent.

Describes methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded.


Applied Biosystems (now Life Technologies) has been awarded US Patent No. 8,012,685, "Detection of analytes and nucleic acids."

Mark E. Shannon and David W. Ruff are named as inventors on the patent.

The patent protects methods of detecting at least one analyte and at least one nucleic acid in a sample, as well as reagents for carrying out the methods.


Gen-Probe has been awarded US Patent No. 8,008,066, "System for performing multi-formatted assays."

Gary D. Lair, Thanh N. Nguyen, Haitao Li, Florence F. Li, Byron J. Knight, Robert E. Heinz, Jerzy A. Macioszek, Christopher B. Davis, and Robert F. Scalese are named as inventors on the patent.

Describes an automated analyzer for performing multiple diagnostic assays simultaneously. The analyzer includes stations for "automatically preparing a sample, incubating the sample, performing an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte," the patent abstract states. The patent also covers an automated receptacle-transporting system that moves the sample vessels from one station to the next, as well as a method for performing an automated diagnostic assay that includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.


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