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IP Watch: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Aug 23, 2011


Applied Biosystems (Life Technologies) has been issued US Patent No. 8,005,628, "Systems and methods for baseline correction using non-linear normalization."

Stephen Gunstream is named as inventor on the patent.

Discloses systems and methods for normalizing detected emission data collected in real-time PCR and other reactions. In some embodiments, a sample plate can be loaded with a fluorescent dye and subjected to a real-time PCR reaction. During the initial cycles, detected emissions that correspond to the background signal contributed by the plate, buffer, and other non-reactant pieces of the reaction system and chemistry can be identified. The raw emission data can be normalized by dividing the emission data by the identified baseline signal. According to various embodiments, the normalized amplification profile can normalize to an initial value of 1, because the actual signal emerges from the baseline at the point exponential growth begins. A normalized amplification profile based on a ratio to the baseline can create a more uniformly scaled amplification curve across different samples, filters, wells, dyes, or machines, the patent's abstract states.

Abbott Laboratories has been issued US Patent No. 8,005,625, "Method for analyzing reactions using an information system."

Eric Shain, John Clemens, Tzyy-Wen Jeng, and George Schneider are named as inventors on the patent.

Discloses a method and system for determining the quantity of an analyte initially present in a chemical and or biological reaction; as well as a computer implemented method and system to automate portions of the analysis comprising mathematical or graphical analysis of an amplification reaction.

The University of South Florida has been issued US Patent No. 8,003,778, "Detection of red tide organisms by nucleic acid amplification."

John Paul, Mike Gray, and Erica Casper are named as inventors on the patent.

Describes a real-time reverse transcription PCR, or [nucleic acid sequence-based amplification], method (and associated primers) targeting the rbcL gene for the detection and quantitation of the Florida red tide organism, Karenia brevis.

Icon Genetics has been issued US Patent No. 8,003,381, "Amplification vectors based on trans-splicing."

Victor Klimyuk, Peter Ivanov, Sylvestre Marillonnet, Meinhart Zenk, and Yuri Gleba are named as inventors on the patent.

Provides a process of amplification and/or expression of a sequence of interest in a cell by generating within the cell at least one amplicon by trans-splicing between an RNA sequence designed to be capable of trans-splicing, and a target RNA, whereby the amplicon is capable of amplifying in the cell and expressing a sequence of interest.

The California Institute of Technology has been issued US Patent No. 8,003,370, "Thermal cycling apparatus."

George Maltezos, Matthew Johnston, David Goodwin, Axel Scherer, and Christopher Walker are named as inventors on the patent.

Provides an apparatus for heating and cooling samples in sample vessels having uniform temperature profiles. The apparatus can be used for performing PCR, in particular real-time PCR, with control and uniformity. The system employs a heat block containing a liquid composition to efficiently transfer heat to and from reaction vessels, the patent's abstract states.

Illumina has been issued US Patent No. 8,003,354, "Multiplex nucleic acid reactions."

Min-Jui Shen, Arnold Oliphant, Scott Butler, John Stuelpnagel, Mark Chee, Kenneth Kuhn, and Jian-Bing Fan are named as inventors on the patent.

Discloses a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.

Pacific Biosciences has been issued US Patent No. 8,003,330, "Error-free amplification of DNA for clonal sequencing."

Cheryl Heiner, Stephen Turner, and Kevin Travers are named as inventors on the patent.

Provides methods of producing low copy number circularized nucleic acid variants that can be distributed to reaction volumes. The methods include providing a template nucleic acid; producing a population of clonal nucleic acids from the template nucleic acid; generating a set of partially overlapping nucleic acid fragments from this population; circularizing the partially overlapping nucleic acid fragments to produce circularized nucleic acid variants; and aliquotting the circularized nucleic acid variants into reaction volumes. The patent also provides related compositions of nucleic acid templates.

Samsung Electronics has been issued US Patent No. 8,003,322, "Method of amplifying a target nucleic acid by rolling circle amplification."

Joo-won Rhee, Young-sun Lee, Kak Namkoong, and Chin-sung Park are named as inventors on the patent.

Provides a kit and a method for amplifying a nucleic acid using rolling cyclic amplification, or RCA. The method includes amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA in a reaction solution including two hairpin oligos, a target nucleic acid, a DNA ligase, an endonuclease, a DNA polymerase, and a primer.

Sequenom has been issued US Patent No. 8,003,317, "Methods for high-level multiplexed polymerase chain reactions and homogenous mass extension reactions."

Martin Beaulieu and Dirk Van Den Boom are named as inventors on the patent.

Provides optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provides methods for performing multiplexed amplification of target nucleic acid.