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IP Watch: Life Tech, Bio-Rad, Symphogen, USDHHS, Agilent, Cornell University Win US Patents

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Applied Biosystems (Life Technologies) has been awarded US Patent No. 8,285,492, "System and method for interpolative calibration."

Stephen Gunstream is named as the inventor.

Provides systems and methods for calibrating emission data or other information signals collected during a PCR, amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.


Bio-Rad Laboratories has been awarded US Patent No. 8,285,489, "Multi-stage, regression-based PCR analysis system."

Jeffrey Lerner is named as the inventor.

Provides systems and methods for analyzing data to determine properties of a PCR process or other process exhibiting amplification or growth. Data representing amplification can be distinguished from data representing a jump or other error. A modified sigmoid function containing a drift term may be used in determining the properties. A multi-stage functional fit of the amplification data can provide increased accuracy and consistency of one or more of the properties. A baseline of the amplification data can be determined by analyzing an integrated area of a first derivative function of the data. A reference quantitation value can also be determined from locations of maxima of different derivative functions of the amplification data, e.g., a weighted average of the maxima locations for the second and third derivatives may be used.


Symphogen has been awarded US Patent No. 8,283,294, "Method for cloning cognate antibodies."

Jesper Kastrup, Lars Nielsen, and Per-Johan Meijer are named as inventors.

Relates to a procedure for linking cognate pairs of VH and VL encoding sequences from a population of cells enriched in particular surface antigen markers. The linking procedure involves a multiplex molecular amplification procedure capable of linking nucleotide sequences of interest in connection with the amplification, in particular multiplex PCR. The method is particularly advantageous for generating cognate pair libraries as well as combinatorial libraries of variable region encoding sequences from immunoglobulins. The invention also relates to methods for generating chimeric human/non-human antibodies and expression libraries generated by such methods.


The US Department of Health and Human Services has been awarded US Patent No. 8,283,158, "Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array."

Michael Emmert-Buck, Rodrigo Chuaqui, and Michael Tangrea are named as inventors.

Relates to methods and apparatuses for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, et cetera. Such manipulations particularly include assays and nucleic acid amplification protocols.


Agilent has been awarded US Patent No. 8,283,148, "DNA polymerase compositions for quantitative PCR and methods thereof."

Joseph Sorgi, Reinhold Mueller, Gothami Padmabandu, Nick Roelofs, and Holly Hogrefe are named as inventors on the patent.

Relates to the generation and characterization of archaeal DNA polymerase mutants with deficient 3'-to-5' exonuclease activity and reduced base analog detection activity. The patent also describes such mutants containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of such DNA polymerases.


Cornell University has been awarded US Patent No. 8,283,121, "Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions."

Francis Barany, Matthew Lubin, George Barany, and Robert Hammer are named as inventors.

Relates to a method for identifying a target nucleotide sequence. The method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a PCR mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and PCR permits multiplex detection of nucleic acid sequence differences.

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