Universidad del Pais Vasco of Leioa-Vizcaya, Spain, has been awarded US Patent No. 8,486,637, "Methods and reagents for the detection of Salmonella spp."
Javier Candina, Aitor Ruiz, Joseba Bikandi, Fernando Otsoa, Ilargi Ballesteros, Fernando Aguirre, and Isabel Ruiz de Galarreta are named as inventors.
Relates to an in vitro method for the detection of bacteria of the Salmonella spp. genus by means of quantitative PCR using specific primers for the pathogen from DNA and RNA samples from the microorganism. The method is useful in the detection of viable and non-viable microorganisms of Salmonella spp. in environmental, clinical, and food samples. The invention also relates to a kit used for putting the method into practice.
The California Institute of Technology has been awarded US Patent No. 8,486,636, "Nucleic acid amplification using microfluidic devices."
Markus Enzelberger, Jian Liu, and Stephen Quake are named as inventors.
Provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certain devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions, exposing it to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.
AmberGen has been awarded US Patent No. 8,486,634, "Amplifying bisulfite-treated template."
Mark Lim and Kenneth Rothschild are named as inventors.
Describes methods of amplifying nucleic acid. The method uses primers on solid support, e.g. a population of beads, and involves amplifying a population of nucleic acid template molecules, wherein the nucleic acid template molecules have been treated with bisulfite, creating loaded beads comprising amplified nucleic acid.
Enzo Diagnostics has been awarded US Patent No. 8,486,633, "Method for detecting nucleic acid sequences."
Elazar Rabbani, Jannis Stavrianopoulos, James Donegan, Jack Coleman, and Marleen Walner are named as inventors.
Describes a method for detecting a target nucleic acid sequence by non-linear amplification.
Qiagen has been awarded US Patent No. 8,486,628, "Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides."
Dirk Loeffert, Christian Korfhage, and Holger Engel are named as inventors.
Relates to the normalization of quantification of nucleic acids in samples, such as mixtures of nucleic acids. More specifically, the patent relates to a method for the normalization of the quantity of a specific nucleic acid in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.
Applied Biosystems (Life Technologies) has been awarded US Patent No. 8,486,627, "Methods, compositions, and kits for amplifying and sequencing polynucleotides."
Peter Nien-Tung Ma is named as inventor.
One aspect of the patent provides methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more terminating nucleotides, the incubation being carried out at a first temperature suitable for amplifying the polynucleotide with the processive amplification polymerase. The method also involves incubating the amplification product at a second temperature suitable for forming a plurality of differently-sized extended sequencing primers with the sequencing polymerase; and evaluating the extended sequencing primers in order to determine the sequence of the polynucleotide. The reactions at the first and second temperatures can be carried out in a single reaction vessel. The patent also provides compositions and kits for carrying out the methods.