Bioneer has been awarded US Patent No. 8,513,399, "Primers for PCR amplification comprising basic parts within the primer sequences."
Hyun Bae Kim, Seong Youl Kim, Jun Mo Gil, Hae Joon Park, and Han Oh Park are named as inventors.
Relates to primers for PCR amplification comprising basic parts within the primer sequences, and a method of PCR amplification using the same. More precisely, the invention relates to primers capable of amplifying different templates and having basic parts complementary to mutated sites or polymorphic sites of template DNA. The method for PCR amplification comprises the steps of mixing the composition including the primers for PCR amplification with nucleic acid template, and performing PCR with the mixture. The primers contain basic parts not having specific coding information in their nucleotide sequences, so that they can simultaneously amplify different templates having mutated sites.
NuGen Technologies has been awarded US Patent No. 8,512,956, "Method for clonal amplification."
Nurith Kurn is named as the inventor.
Provides methods, libraries, and kits for the archiving and clonal amplification of sequences related to target polynucleotide sequences. The method allows for the generation and attachment of polynucleotides with defined 3' and 5' ends to solid surfaces. The polynucleotides attached to the solid substrates can be stored or archived as libraries and can subsequently be retrieved for analysis, for example by clonal amplification using a single composite amplification primer comprising a DNA portion and an RNA portion. In some embodiments, nucleotides attached to solid surfaces can be used for sequencing nucleotide sequences related to the target DNA. The methods are applicable to total RNA and/or total DNA analysis.
Gen-Probe (Hologic) has been awarded US Patent No. 8,512,955, "Methods and compositions for nucleic acid amplification."
Steven Brentano, Dmitry Lyakhov, Norman Nelson, James Carlson, Michael Becker, and Lyle Arnold, Jr., are named as inventors.
Describes compositions, reaction mixtures, and methods for performing an amplification reaction, including a multiplex amplification reaction. The method uses one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, which is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
Molecular Detection has been awarded US Patent No. 8,512,954, "Methods, compositions, and kits for detection and analysis of antibiotic-resistant bacteria."
Yosef Paitan is named as the inventor.
Relates generally to detection of antibiotic-resistant bacteria in a sample. In particular, the invention provides methods, compositions, and kits for detecting and analyzing methicillin-resistant Staphylococcus aureus and other methicillin-resistant bacteria in a sample. A specific claim of the invention provides a first, second, and third set of primers to certain described polynucleotide sequences; combining the primers with a sample in a reaction mixture; performing a multi-cycle amplification reaction with the reaction mixture; determining cycle numbers of appearance of each of the mecA, MSSA-orfX and S. aureus-specific polynucleotide sequences, wherein said cycle numbers indicate whether MRSA is present in a sample.
Quest Diagnostics has been awarded US Patent No. 8,512,948, "Compositions and methods for detecting mutations in JAK2 nucleic acid."
Maher Albitar and Wanlong Ma are named as inventors.
The invention is based on the identification of novel mutations in the JAK2 gene and JAK2 protein, and provides compositions and methods useful for diagnosing hematopoietic diseases including, for example, myeloproliferative diseases. The invention also provides compositions and methods useful for determining the prognosis of an individual diagnosed as having a hematopoietic disease.
Kurashiki Boseki Kabushiki Kaisha of Kurashiki-shi, Japan, has been awarded US Patent No. 8,511,482, "Method of stably producing membrane and use thereof in method of separating and purifying nucleic acid."
Hideaki Tanaka and Yoshihiko Makino are named as inventors.
Describes a method of producing a porous membrane. The method comprises casting a polymer solution, in which a polymer is dissolved in a mixture of a good solvent, a poor solvent, and a non-solvent, over a support, so as to form a casted polymer solution; drying the casted polymer solution, so as to form a cast film; and subjecting the cast film to a phase separation, wherein the porous membrane is produced under a condition where a temperature of a casted surface is lower than a temperature of the polymer solution, and each temperature change of the polymer solution and the casted surface is kept within 3 degrees Celsius.