Ibis Biosciences (Abbott) has been awarded US Patent No. 8,163,895, "Compositions for use in identification of orthopoxviruses."
Rangarajan Sampath, Thomas Hall, David Ecker, and Steven Hofstadler are named as inventors on the patent.
Provides oligonucleotide primers, and compositions and kits containing the same, for rapid identification of orthopoxviruses by amplification of a segment of viral nucleic acid followed by molecular mass analysis.
The University of Utah has been awarded US Patent No. 8,163,566, "Microporous materials, methods of making, using, and articles thereof."
Roger Smith, Karl Voelkerding, Marc Elgort, and Jacob Durtschi are named as inventors on the patent.
Describes methods for separating one or more analytes present in a fluid sample. The methods involve passing the fluid through or into a microporous material, wherein the analytes are localized near the surface of the microporous material. Additional processing steps such as hybridization and amplification can be performed once the analyte is localized. In one method, once the analyte is localized, it can be detected, counted, and correlated in order to determine its concentration in the sample. In another method, the localized analyte is destabilized to make it more accessible for chemical manipulation. The patent also discloses modified microporous materials and composite materials that can be used in any of the described methods and articles; and various kits and articles such as filtration devices containing the microporous materials.
Fluidigm has been awarded US Patent No. 8,163,492, "Microfluidic device and methods of using same."
Marc Unger, Ian Manger, Michael Lucero, Yong Yi, Emily Miyashita-Lin, Anja Wienecke, and Geoffrey Facer are named as inventors on the patent.
Describes a variety of elastomeric-based microfluidic devices, and methods for using and manufacturing such devices. Certain of the devices have arrays of reaction sites to facilitate high-throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping, and gene expression analyses.
Sysmex of Hyogo, Japan, has been awarded US Patent No. 8,163,490, "Method of analyzing methylated DNA."
Ayako Sakai, Masahiro Kajita, and Hideki Ishihara are named as inventors on the patent.
Provides a method of analyzing methylated DNA. The method comprises the steps of: (A) treating a DNA-containing sample with a restriction enzyme to obtain a sample containing a DNA fragment; (B) concentrating methylated DNA contained in the sample obtained in step (A) to obtain a methylated DNA concentrate; (C) subjecting the methylated DNA concentrate obtained in step (B) and a primer set to a nucleic acid amplification reaction, wherein the primer set performs the nucleic acid amplification reaction in step (C) by using a template DNA that does not have a CpG site; (D) detecting an amplification product obtained in step (C); (E) judging whether the methylated DNA concentrate obtained in step (B) is appropriate as a sample for detecting methylated DNA, on the basis of the detection result of the amplification product in step (D); and (F) analyzing the methylated DNA contained in the methylated DNA concentrate.
Vandalia Research has been awarded US Patent No. 8,163,489, "Method for a continuous rapid thermal cycle system."
Elizabeth Murray, Derek Gregg, Michael Norton, Justin Swick, and William Towler are named as inventors on the patent.
Discloses an efficient, high-speed production-scale synthesis method for high-molecular-weight organic substances, such as DNA. The invention includes a method of conducting PCR, which comprises transporting a liquid through polymeric tubing disposed through a first reaction cycle region and at least a second reaction cycle region. Each region comprises at least a first and a second temperature zone, the temperature in each zone of each region being substantially identical to the corresponding first and second zones in the first region. The liquid is an aqueous solution comprising PCR reactants and a surface absorbing polymer.
Roche Molecular Systems has been awarded US Patent No. 8,163,484, "Light emission modifiers and their uses in nucleic acid detection, amplification, and analysis."
Amar Gupta and Stephen Will are named as inventors on the patent.
Relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real-time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. The methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. The patent also describes use of these light emission modifiers in background reduction, nucleic acid duplex stabilization, and other applications; and describes related kits, reaction mixtures, and integrated systems.
Quest Diagnostics and US Genomics have been awarded US Patent No. 8,163,480, "Nucleic acid size detection method."
Donghui Huang, Charles Strom, Steven Potts, and Jenny Rooke are named as inventors on the patent.
Provides methods of determining the size of a particular nucleic acid segment of interest in a sample of nucleic acids through fragmentation of DNA, size fractionation, an optional second fragmentation, and identification using a marker sequence. In particular aspects, an expansion or reduction of tandem repeat sequences can be detected. In further aspects, carriers and individuals afflicted with fragile X syndrome or other diseases associated with tandem repeats can be distinguished from normal individuals.