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IP Update: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Jun 16, 2010

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Gen-Probe has been awarded US Patent No. 7,739,054, "Method and algorithm for quantifying polynucleotides."

James Carrick, Jeffrey Chismar, and Michael Gilly are listed as inventors on the patent.

According to its abstract, the patent discloses a machine-executable method of analyzing growth curve data to identify the transition from a baseline phase into a growth phase. Applications of the method include analysis of results from time-dependent monitoring of amplicon synthesis in a nucleic acid amplification reaction to quantify a starting amount of a nucleic acid template in a test sample. The method advantageously simplifies the quantification by circumventing the need to establish thresholds used for calculating initiation of the growth phase, to calculate derivatives, or to perform linear regression analysis.


Enzo Life Sciences has been awarded US Patent No. 7,737,281, "Purine-based fluorescent dyes."

Maciej Szczepanik is the sole inventor listed on the patent.

According to its abstract, the patent describes novel purine-based fluorescent dyes that may be used for staining, localizing, and otherwise labeling target molecules, such as nucleic acids, for detection, amplification, and quantification.


Hitachi has been awarded US Patent No. 7,737,268, "Method of recovering nucleic acids and kit for recovering nucleic acids."

Norihito Kuno and Kenko Uchida are listed as inventors on the patent.

The patent describes a method of separately recovering DNA and RNA from a biological sample solution. The molecules are separated and recovered from a single biological sample by controlling pH values, temperature, and/or concentration of cationic ions of a nucleic acid sample solution containing a caotropic agent.


Life Technologies has been awarded US Patent No. 7,736,874, "Methods for cloning amplified nucleic acid molecules."

Donna Fox and Deb Chatterjee are the inventors listed on the patent.

According to its abstract, the patent covers methods to facilitate the cloning of nucleic acid molecules. In particular, the invention relates to the use of polymerase inhibitors, including but not limited to anti-polymerase antibodies (such as anti-Taq antibodies) and fragments thereof, to inactivate residual polymerase activity remaining after the amplification (particularly via PCR) of a target nucleic acid molecule. The patent also provides compositions — particularly storage-stable compositions, comprising one or more components, such as one or more restriction endonucleases and one or more polymerase inhibitors — that are useful in cloning amplified or synthesized nucleic acid molecules by the above-described methods. The invention also relates to nucleic acid molecules produced by the methods, and to genetic constructs (such as vectors) and host cells comprising the nucleic acid molecules.


Hologic has been awarded US Patent No. 7,736,854, "Methods of detection of a target nucleic acid sequence."

Joseph Sorge is the sole inventor listed on the patent.

The patent discloses methods of generating a signal indicative of the presence of a target nucleic acid in a sample. The methods involve forming a cleavage structure comprising duplex and single-stranded nucleic acid by incubating a sample comprising a target nucleic acid with a thermostable nucleic acid polymerase substantially lacking 5' to 3' exonuclease activity, and cleaving said cleavage structure with a thermostable Fen nuclease lacking 5' to 3' synthetic activity, to generate a signal.


Seoulin Bio Science and the Seoul National University Industry Foundation have been awarded US Patent No. 7,732,589, "Primers for amplifying hsp65 gene of mycobacterial species, hsp65 gene fragments, and method of identifying mycobacterial species with the same."

Bum-Joon Kim, Yoon-Ho Kook, and Jeong-Mi Kim are the inventors listed on the patent.

The patent relates to a pair of primers specific to mycobacterial species, a polynucleotide of an hsp65 gene fragment, and a method for identifying mycobacterial species using the same. More specifically, the 604-base-pair hsp65 gene fragment can be applied to identification methods of mycobacteria such as comparative sequence analysis, probe hybridization, and PCR-RFLP, which can resolve the problems of conventional identification methods based on biochemical characteristics, where the genus Mycobacterium covers various species and has a low growth rate; and of the problems of 16s rDNA.


Pall Genesystems has been awarded US Patent No. 7,732,136, "Device for thermo-dependent chain reaction amplification of target nucleic acid sequences, measured in real-time."

Gabriel Festoc is the sole inventor listed on the patent.

The patent covers a device for amplifying target nucleic acids, reaction cartridges for use in the device, and modes of use of the device.

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