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IP Update: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Jun 23, 2010

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Gen-Probe and BioMérieux have been jointly awarded US Patent No. 7,741,027, "Amplification of HIV-1 sequences for detection of sequences associated with drug-resistance mutations."

Yeasing Yang, Steven Brentano, Odile Babola, Nathalie Tran, and Guy Vernet are named as inventors on the patent.

According to its abstract, the patent discloses sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences. The patent also discloses methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using the amplification oligonucleotides specific for gag and pol target sequences.


Ibis Biosciences (now part of Abbott) has been awarded US Patent No. 7,741,036, "Method for rapid detection and identification of bioagents."

David Ecker, Richard Griffey, Rangarajan Sampath, Steven Hofstadler, and John McNeil are named as inventors on the patent.

Discloses a method for detecting and identifying unknown bioagents, including bacteria, viruses, and the like, by a combination of nucleic acid amplification and molecular weight determination using primers that hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a base composition signature that is then matched against a database of base composition signatures, by which the bioagent is identified.


Genera Biosystems has been awarded US Patent No. 7,741,459, "Nucleic acid anchoring system comprising covalent linkage of an oligonucleotide to a solid support."

Karl Poetter and Brendan Toohey are named as inventors on the patent.

Provides an anchoring system generally comprising a solid support and a chemical linking moiety useful for ether formation with another chemical moiety on a nucleic acid molecule. The patent further discloses methods for anchoring a nucleic acid molecule to a solid support via a covalent linkage. The anchoring system can be used to construct nucleic acid arrays, to purify nucleic acid molecules, and to anchor nucleic acid molecules so that they can be used as templates for in vitro transcription and/or translation experiments and to participate in amplification reactions. The invention is particularly adaptable for use with microspheres and to the preparation of microsphere suspension arrays and optical fiber arrays. The anchoring system permits the generation of an anchored oligonucleotide for use as a universal nucleic acid conjugation substrate for any nucleic acid molecule or population of nucleic acid molecules. The invention further provides a kit useful for anchoring nucleic acid molecules or comprising nucleic acid molecules already anchored to a solid support.


Innogenetics, Enterprise Ireland, and the National University of Ireland in Galway have been jointly awarded US Patent No. 7,741,461, "Nucleic acid probes and methods for detecting clinically important fungal pathogens."

Terry Smith, Majella Maher, Cara Martin, Geert Jannes, Rudi Rossau, and Marjo Van der Weide are named as inventors on the patent.

Relates to detecting and identifying clinically important fungi. More particularly, the present invention relates to species-specific probes originating from the internal transcribed spacer region of rDNA to detect fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cryptococcus neoformans, and Pneumocystis carinii in clinical samples, and methods using said probes.


Illumina has been awarded US Patent No. 7,741,463, "Method of preparing libraries of template polynucleotides."

Niall Gormley, Geoffrey Smith, David Bentley, Roberto Rigatti, and Shujun Luo are named as inventors on the patent.

Provides a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5' ends and at their 3' ends.