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IP Update: Recent Patents Related to PCR, Nucleic Acid Amplification, and Sample Prep: Jul 15, 2010

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Zymo Research has been issued US Patent No. 7,754,873, "Isolation of nucleic acid using colored buffers."

Xiyu Jia and Linda Jia are named as inventors on the patent.

Describes isolation of plasmid DNA from bacteria. The addition of dyes to alkaline lysis-based purification buffers P1, P2, and P3 allows for improved visual monitoring of the steps of preparing a bacterial lysate filtrate coupled to filtration or spin-column chromatography. The method comprises suspending the bacterial cells with buffer P1 (suspension is red/pink); lysing the bacteria with buffer P2 (suspension goes from red/purple color to translucent/purple); precipitating cellular debris with buffer P3 (solution becomes yellow with debris suspension); centrifuging or filtering to product a lysate filtrate; binding the lysate filtrate to a DNA binding matrix; washing; and isolating the plasmid via chromatography. The yield and quality of plasmid DNA is improved due to more consistent lysis. Errors in buffer addition are reduced by visualizing the color as buffers are added and also of changes in color of the preparation at each step.


Life Technologies has been issued US Patent No. 7,754,453, "Methods of using FET labeled oligonucleotides that include a 3'-5' exonuclease-resistant quencher domain and compositions for practicing the same."

Quin Chou and Dragan Spasic are named as inventors on the patent.

Provides methods and compositions for detecting a primer extension product in a reaction mixture. A primer extension reaction is conducted in the presence of a polymerase having 3'-to-5' exonuclease activity and at least one fluorescence energy transfer-labeled oligonucleotide probe that includes a 3'-to-5' exonuclease-resistant quencher domain. The patent also provides systems and kits for practicing the subject methods. The invention is useful in a variety of applications, according to the patent, and is particularly suited for use in high-fidelity PCR-based reactions, including SNP detection applications, allelic variation detection applications, and the like.


Samsung Electronics has been issued US Patent No. 7,754,452, "Polymerase chain reaction module and multiple-polymerase chain reaction system, including the module."

Jin-tae Kim, Kak Namkoong, Kwang-wook Oh, Chin-sung Park, and Yu-jin Seo are named as inventors on the patent.

Provides a PCR module and PCR system including the same. The PCR module includes: a detachable PCR chip including a chamber unit in which a solution is accommodated; a heater unit for heating the solution in the chip with a preset temperature; a detecting unit for detecting a signal of the solution; a chip installation unit for mounting/detaching the chip using a one-touch method, in which the heater unit is adhered to the chip with a predetermined pressure when mounting the chip and the heater unit is separated from the chip when detaching it; and a housing covering at least the heater unit and the detecting unit so that they are not exposed to the outside.


Affymetrix has been issued US Patent No. 7,754,451, "Multiplex oligonucleotide addition and target amplification."

Malek Faham and Jianbiao Zheng are named as inventors on the patent.

Describes methods for appending oligonucleotides directly to nucleic acid templates, particularly to defined sites internal to single-stranded templates. Appending first and second common priming sites to each of a plurality of templates of distinct sequence allows the subsequent stoichiometric amplification of the templates.

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