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European Researchers Enable Single-Dye Multiplex qPCR via Melt-Curve Analysis in Each Thermal Cycle

Jun 24, 2015
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Madeleine Johnson
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NEW YORK (GenomeWeb) – Multiplex qPCR typically requires a separate fluorophore and optical channel for each target in a sample, but a proof-of-principle methods paper has now described a way to multiplex using only a single dye by embedding melt analysis within the PCR thermal cycling itself.

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