Common Digital PCR Sample Prep Step Could Cause False Positive Mutation Detection

Apr 22, 2016

|

Premium

NEW YORK (GenomeWeb) – The use of high temperature to fragment genomic DNA prior to digital PCR analysis has been shown to induce mutations that are detected as false-positive results for some rare alleles, according to newly published research.

Digital PCR demands fragmentation of DNA in order to ensure uniform droplet formation. The methods used to accomplish this include restriction digestion, microwaving irradiation, biopolymer-based shredding, acoustic shearing, and treatment with high heat.

Get the full story with
GenomeWeb Premium

Only $95 for the
first 90 days*

GenomeWeb Premium gives you:
✔ Full site access
✔ Interest-based email alerts
✔ Access to archives

Never miss another important industry story.

Try GenomeWeb Premium now.

You may already have institutional access!

Check if I qualify.

Already a GenomeWeb or 360Dx Premium member?
Login Now.

*Before your trial expires, we’ll put together a custom quote with your long-term premium options.

Not ready for premium?

Register for Free Content
You can still register for access to our free content.

Breaking News

The Scan

Viral Genomes Back in Time

Researchers uncovered the HIV virus within a tissue sample collected in 1966, the Atlantic reports.

To Give a Microbiome?

Nature News reports there are a handful of clinical trials underway to evaluate vaginal microbiome seeding of newborns born via caesarian section.

The End of the Cave Bears

The Washington Post writes that humans may have contributed to the extinction of cave bears some 20,000 years ago.

PLOS Papers Tackle Trypanosomiasis Susceptibility, Diabetes Modulators, More

In PLOS this week: gene variant may protect against trypanosomiasis, GLIS3 role in type 2 diabetes, and more.

Featured White Papers

Sample Normalization with RNAGEM

RNAGEM - mRNA Profiles as Close to Biological Reality as Possible

Cell-Based Therapies – Be on the Safe Side

Poster: Next Generation Cellular Therapeutic Technologies — Rapid Detection of Bacterial Contamination

Sep

16

Featured Webinar

Using Next-Generation Sequencing to Detect Genomic Mutations in Hematologic Malignancies

This webinar will discuss a next-generation sequencing approach for detecting genomic mutations in hematologic maglignancies.

Sep

18

Featured Webinar

Highly Sensitive Structural Variant Detection for Medical Genetics: A Comparison to the Standard of Care

This webinar will outline how a team at Radboud University Medical Center is assessing ultra-long read optical mapping on the Bionano Saphyr system to replace classical cytogenetics approaches in routine testing and for the discovery of novel structural variants with potential scientific, prognostic, or therapeutic value that are missed by standard approaches.

Oct

23

Featured Webinar

Single-Cell Methylation Profiling of Pediatric Tumors via a Unique NGS-based Approach

This webinar will illustrate how single-cell methylation sequencing can be applied to gain significant insight into epigenetic heterogeneity in disease states, advancing cancer research discoveries.

Oct

24

Featured Webinar

Designing, Decoding & Delivering on Genetic Testing for Inherited Disease at Versiti

This webinar will tell the story of Versiti’s journey in transforming genetic testing from a manual to a digitized process. It will include detail on how the organization succeeded, pain points along the way, a novel approach to variant assessment, and future plans for the program.

Menu