Common Digital PCR Sample Prep Step Could Cause False Positive Mutation Detection

Apr 22, 2016

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NEW YORK (GenomeWeb) – The use of high temperature to fragment genomic DNA prior to digital PCR analysis has been shown to induce mutations that are detected as false-positive results for some rare alleles, according to newly published research.

Digital PCR demands fragmentation of DNA in order to ensure uniform droplet formation. The methods used to accomplish this include restriction digestion, microwaving irradiation, biopolymer-based shredding, acoustic shearing, and treatment with high heat.

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