Life Technologies said this week that it has developed the RapidFinder Equine ID kit in collaboration with the Instituto de Medicina Genómica.
The new kit detects the presence of equine DNA extracted from meat samples, and is optimized for use in all Applied Biosystems real-time PCR instruments.
The kit comprises all reagents necessary for 48 reactions, and offers a detection limit of 0.01 percent (w/w) in fresh meat. The kit includes a positive control consisting of 0.1 percent horse DNA. It also includes a quantification kit with a limit of 0.05 percent (w/w) and includes the standard for quantification.
RapidFinder Equine ID is available immediately in Europe, Middle East, and Africa via Life Tech's distributors according to local regulatory requirements.
EnviroLogix has introduced the DNAble Salmonella Rapid Screening Kit for live poultry production.
DNAble is a rapid, highly specific isothermal nucleic acid amplification technology that can amplify DNA targets with single-base resolution a billion-fold in 5 to 10 minutes at a constant temperature. A nicking enzyme and a strand-displacing polymerase are used to generate small pieces of DNA that feed a DNA extension reaction that consists of alternating cycles of nicking and extension processes leading to exponential amplification.
According to the company, DNAble is faster than current PCR methods, and offers at least comparable sensitivity and specificity. Unlike PCR, DNAble shows little inhibitory effect from a crude extraction or exogenous DNA. As a result, it allows for highly sensitive detection with minimal to no sample preparation, the company said.