The team reported that engineering a hairpin secondary structure onto the spacer region of sgRNAs can increase specificity by several orders of magnitude.
CRISPR technology has made its way around the world, but in the wake of the He Jiankui controversy, the industry is asking what recourse it has against misuse.
In Nucleic Acids Research this week: microRNAs influence cell type transitions in triple-negative breast cancers, oxidative DNA damage patterns in yeast, and more.
Its most advanced gene expression signatures are for infectious disease diagnostics and are aimed at enabling the appropriate prescription of antiviral and antibiotic medications.
The study authors cautioned that immunological signatures cannot be predicted from tumor mutational burden or microenvironment analysis of a single biopsy alone.
