Although the developers note that the protocol particularly benefits the sequencing of AT-rich genomes, they say that it is also quicker than the standard method, which requires PCR amplification, and "should be used routinely to prepare libraries for Genome Analyzer sequencing."
Using gene expression and copy number analyses of dozens of endometrial cancers, an international research team has turned up characteristic expression profiles and gene amplifications linked to poor outcomes.
The company claims that compared to other targeted sequence-enrichment methods, its approach leads to more uniform coverage, or less enrichment bias. RainDance said it plans to offer up to 4,000 amplifications in a single tube later this year.
Both companies are offering users of second-generation sequencing systems new tools to select portions of the genome for targeted resequencing but have optimized their products for different sequencing platforms.
Planned improvements for the SOLiD include a sample prep-automation instrument that is expected to be market-ready towards the end of 2009. Life Tech expects its Genetic Systems unit, home to the CE and SOLiD platforms, to grow in "low single digits" this year.