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New England Biolabs NEBNext Immune Sequencing Kits

New England Biolabs has launched the NEBNext Immune Sequencing Kits, designed for sequencing full-length immune gene repertoires of B and T cells. The kits enable somatic mutation profiling of these cells via the expression of complete antibody chains. They include modular primer sets for the complete V, D, and J segments; full isotype information analysis covering IgM, IgD, IgG, IgA, and IgE; and TCR chain characterization. They also include a bioinformatic workflow using the Galaxy platform, based on the open-source pRESTO toolkit. Users may convert PCR copies derived from individual molecules into consensus sequences via template-switching Unique Molecular Identifier-based mRNA barcoding.