Illumina Nextera DNA Flex
Illumina has launched its Nextera DNA Flex, a whole-genome sequencing library preparation product that eliminates the need for sample prep of whole blood and saliva, and cuts down on steps in the library prep workflow. The new product utilizes On-Bead Tagmentation, which reduces total library prep turnaround time to less than three hours, the firm said. Among the applications for Nextera DNA Flex are sequencing of human or other large or complex genomes, amplicons, and microbial, parasitic, and fungal species, Illumina added.
Cellecta Human and Mouse Genome-Wide CRISPRa and CRISPRi Libraries, and Mouse CRISPR Knockout Library
Cellecta has added CRISPRa and CRISPRi genome-wide, human and mouse pooled screening libraries to its portfolio of CRISPR products and services. Cellecta has also supplemented its current portfolio of CRISPR gene knockout libraries by adding a Mouse Genome-Wide CRISPR Knockout Library. These new additions to the company's CRISPR portfolio enable researchers to study enhanced gene activation or gene inhibition without modifying genomic DNA, Cellecta said. The gene-regulating CRISPRa and CRISPRi variations make use of an altered CRISPR protein to enhance or inhibit gene expression, rather than inducing mutational changes in a gene's coding sequence. Access to these new libraries provide researchers with new tools to identify potential drug targets or biomarkers, and elucidate mechanisms of drug action.
Key features of the new Cellecta CRISPRa and CRISPRi libraries include single-module format, with each library expressing some 106,000 sgRNAs (human) and 109,000 sgRNAs (mouse) averaging five sgRNAs per gene promoter region; CRISPRa and CRISPRi libraries targeting approximately 19,000 human genes and almost 20,000 mouse genes; and approximately 4,000 non-targeting sgRNA controls.
The new CRISPR Mouse Genome-Wide Knockout Library, based on the canonical CRISPR/Cas9 system, extends knockout screening capability to mouse model systems. The Cellecta CRISPR mouse knockout library targets all mouse protein-coding genes and features approximately 80,000 constructs comprise the single-module library, four sgRNAs target functional domains and conserved transcript regions in each of 19,600 mouse genes, and complete panel of controls include standard, non-targeting controls.
DNAnexus CloudSeq
DNAnexus this week launched its DNAnexus CloudSeq solution, a suite of cloud-based tools and professional services specifically designed to support the informatics infrastructure needs of customers using Illumina's NovaSeq sequencing systems. The CloudSeq solution includes full access to the DNAnexus Platform, a fixed pricing model, and on-demand cloud-enablement and bioinformatics support to help laboratories securely scale operations in as little as four weeks, the firm said.
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