Bio-Rad Droplet Digital PCR-Based Assays
Bio-Rad Laboratories has launched droplet digital PCR Genome Edit Detection assays, which can characterize edits generated by CRISPR-Cas9 or other genome editing tools. The assays use ddPCR technology which increases the signal-to-noise ratio, allowing users to quantify extremely rare edits, even those with frequencies of 0.5 percent and from as little as 5 nanograms of genomic DNA. Users can expect to obtain results within one day.
Children's Hospital Los Angeles OncoKids
Children's Hospital Los Angeles has launched OncoKids, a next-generation sequencing-based panel that detects mutations, gene amplification, and gene fusions for a full range of pediatric cancers including leukemias, bone and soft tissue tumors, and brain tumors. It only requires a small amount of DNA and RNA (20ng) isolated from fresh, frozen, or formalin-fixed paraffin-embedded tissue. The test was developed in collaboration with Thermo Fisher Scientific and uses the Ion Torrent S5 sequencing platform and Ion AmpliSeq technology.
Thermo Fisher Scientific ISQ EC Single Quadrupole Mass Spectrometer, MabPac RP 1mm Columns
Thermo Fisher Scientific has launched a new mass spectrometer and new chromatography columns. The ISQ EC Single Quadrupole mass spectrometer integrates with existing ion chromatography and HPLC systems, and the Chromeleon Chromatography Data Software has instrument control for the new mass spec embedded. The MabPac RP 1mm columns are designed for analyzing monoclonal antibodies, fragments, variants, antibody drug conjugates, and proteins using high-performance reversed-phase chromatography. They can operate with small sample volumes at low flow rates for direct injection into a mass spectrometer.
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