Twist Bioscience NGS Methylation Detection System
Twist Bioscience has launched the Twist NGS Sequencing Methylation Detection system, a sample preparation and target enrichment solution for identifying methylated regions in the human genome.
The system uses EM-seq from New England Biolabs, an enzyme-based alternative to traditional bisulfite sequencing methods that enables preparation of enzymatically converted libraries. The system also includes Twist methylation enhancer, universal blocker and FastHyb, and custom methylation panels that include DNA probes to capture all four potential sequences at a given site: methylated, unmethylated, sense and antisense.
The products can be used in liquid biopsy cancer analysis and epigenetic studies.
New England Biolabs Artic Products for SARS-CoV-2 Sequencing
New England Biolabs has launched three kits based on the Artic Network protocols for multiplexed amplicon-based sequencing of viral RNA: NEBNext Artic SARS-CoV-2 Library Prep Kit and NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit, both for Illumina sequencing; and NEBNext Artic SARS-CoV-2 Companion Kit for Oxford Nanopore Technologies sequencing.
The Artic network is an international research consortium that has developed protocols for the epidemiological monitoring of viral genomes to provide insights into virus evolution and transmission. All three NEBNext Artic SARS-CoV-2 kits yield uniform, ample amplicons from gRNA across a wide copy number range, and use the same RT step for all inputs, NEB said. The two kits for Illumina sequencers provide options for library insert size and downstream sequencing parameters.
Promega LMR MSI Analysis System
Promega has launched its LMR MSI Analysis system, a PCR-based research tool with novel markers for detecting microsatellite instability. The panel consists of eight total markers: four gold-standard markers (BAT-25, BAT-26, MONO-27, and NR-21) and four novel long mononucleotide repeat markers (BAT-52, BAT-56, BAT-59, and BAT-60). According to the company combining these marker sets expands MSI detection ability in difficult samples while maintaining overlap with data gathered using traditional markers. Promega said that the assay has been optimized to enable strong and balanced amplification even from degraded samples such as formalin-fixed, paraffin-embedded tissue.
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