PerkinElmer NEXTflex Unique Dual Index Barcodes
PerkinElmer has added NEXTflex unique dual index barcodes to its NEXTflex family of library prep products. These 192 barcoded adapters provide data security in sequencing applications for use on Illumina platforms. This system is designed to mitigate index hopping and signal spreading, a phenomenon associated with multiplexing. Instruments utilizing a patterned flow cell and exclusion amplification technology experience increased levels of sample misassignment as a result. These adapters address the important issue of sample misidentification, thereby enabling assurance of data integrity, the company said. By allowing researchers to pool multiple libraries in a single flow cell lane, multiplexing with the NEXTflex barcoded adapters also increases processing capacity, while reducing costs.
Phase Genomics, Pacific Biosciences FALCON-Phase
Phase Genomics and Pacific Biosciences have launched genome assembly software that combines PacBio single-molecule sequencing data with Hi-C scaffolding technology to assemble phased genomes. The FALCON-Phase software is open source and available as a service through Phase Genomics.
Asuragen AmplideX PCR/CE DMPK Kit
Asuragen has launched the AmplideX PCR/CE DMPK Kit, a research-use-only assay designed to simplify analysis of repeat expansions in the DMPK gene, which is implicated in myotonic dystrophy type 1 (Steinert's disease). The repeat expansions in DMPK commonly extend beyond 1,000 CTG repeats and therefore have previously required the use of Southern blot technology to estimate their size, Asuragen noted, adding that its new kit is a significant improvement over this process.
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