Pacific Biosciences Sequel System 6.0
Pacific Biosciences has launched updates to its Sequel sequencing system, including software version 6.0, reagents version 3.0, and SMRT cells 1M v3. Together, these changes, which are part of the Sequel System 6.0 release, result in sequence reads with greater than 99 percent accuracy, throughput of up to 50 gigabases per SMRT cell, and average read lengths of up to 100 kilobases, depending on the insert size, the firm said. For amplicon sequencing and RNA sequencing, customers can generate up to 500,000 reads with single-read accuracy greater than 99 percent. For whole-genome sequencing, customers can generate up to 20 gigabases of data per SMRT cell, with average read lengths of up to 30 kilobases.
Oxford Nanopore MinIT
Oxford Nanopore Technologies has launched the MinIT, a compute module for use with the MinIon sequencer. MinIT is equipped with a Nvidia AGX system for real-time high-throughput data analysis and is preconfigured with software for MinIon set up and operation. Users can operate the module using either a tablet or mobile phone. MinIT runs both the instrument software MinKnow and the real-time analysis platform for nanopore data EPI2ME. Customers can power the tool using battery packs, which are available separately.
Cellecta CloneTracker XP Expressed Lentiviral Barcode Library and Barcoded CRISPR Library
Cellecta has launched the CloneTracker XP Expressed Lentiviral Barcode Library and CloneTracker XP Barcoded CRISPR Library product lines. The new CloneTracker XP Barcode Libraries differ from Cellecta's standard CloneTracker Barcode Library in that the unique DNA sequence is designed to express on an RNA transcript in the cells. The libraries can therefore be detected by either DNA or RNA sequencing. Researchers can use these libraries to label several million cells each with a unique barcode, subsequently performing NGS to sort out sub-populations of progeny cells derived from the original progenitors at any point during the experiment.
Additionally, a variation of the new CloneTracker XP barcode labeling product introduces a gene effector, in this case CRISPR sgRNA, into the barcode library. Each effector targets and disrupts a specific gene in each of the cells that pick up a barcode. In combination with cell-specific barcode tracking, this knockout helps researchers see how specific genetic disruptions change the cells' characteristics while simultaneously identifying the genetic pathways that are activated to produce them. The firm offers two small, pre-made CloneTracker XP Barcoded CRISPR knockout libraries targeting 27 human and mouse anti-cancer genes, as well as custom library development services for CloneTracker XP Barcoded CRISPR Libraries. The CloneTracker XP Expressed Barcode Libraries are available with barcodes expressed in the 3′- or 5′-UTR of an RNA transcript, and with fluorescent or chemiluminescent reporters.
For more new products and services, please visit the New Products page on our website.