Oxford Nanopore Technologies Direct cDNA (SQK-DC109), PCR-cDNA (SQK-PCS109) Kits
Oxford Nanopore Technologies has launched two new cDNA sequencing kits for its nanopore devices. The kits, called Direct cDNA (SQK-DC109) and PCR-cDNA (SQK-PCS109), generate sequence data for full-length cDNA and require less than five hours of sample preparation. The Direct cDNA kit does not require PCR and needs 100 ng of poly-A+ RNA. The PCR-cDNA kit has an input requirement of just 1 ng of polyA+ RNA and can start from total RNA. Users can obtain 10 to 15 million reads on a MinIon flow cell and more than 60 million reads on a PromethIon flow cell. According to the firm, the kits are available for the MinIon, GridIon, and PromethIon sequencers.
Illumina NovaSeq 6000 S Prime Reagent Kits
Illumina has launched its S Prime Reagent Kits for the NovaSeq 6000 next-generation sequencing instrument. The kits, which are available in 100-, 300-, and 500-Cycle configurations, enable paired-end reads as long as 250 bases. They require run times of 13 hours for 50-base paired end reads and up to 38 hours for 250-base paired end reads. Illumina said that total throughput is between 65 and 400 gigabases, depending on the read length.
Fluidigm REAP-Seq Protocol for C1 System
Fluidigm has introduced a REAP-seq (RNA expression and protein sequencing) protocol for use with its C1 sample prep system for single-cell genomics. C1 REAP-seq enables characterization of unique cellular subtypes and functional states by measuring the expression of both cellular proteins and RNAs. Users can pair the protocol with functional imaging assays that measure differences in cell size, morphology, or phenotype within the clear C1 microfluidic cell chambers. The firm developed C1 REAP-seq in collaboration with Merck for co-detection of both cellular protein and RNA using microfluidic technology.
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