Lexogen Corall Whole Transcriptome Library Kit
Lexogen has launched its Corall Total RNA-Seq Library Prep Kit. The firm said that researchers can use the kit for all whole-transcriptome analysis applications, including differential expression, isoform quantification, and single nucleotide polymorphism and mutation detection. Lexogen highlighted that Corall can use as little as 1 nanogram and up to 1 microgram of total RNA. The kit integrates unique molecular identifiers that facilitate the identification and removal of PCR duplicates, while ensuring high strand specificity. The kits include up to 96 i7 indices for sample multiplexing. Researchers can also add i5 indices for a total of 9,216 indexing combinations. The firm offers the kits in in 24 and 96 prep sizes, as a standalone library preparation kit, or in combination with with the RiboCop rRNA Depletion Kit.
Bionano Genomics Saphyr Chip, Sample Prep, Data Analysis
BioNano Genomics this week released new products related to its Saphyr platform for structural variation detection in genome analysis. First, the company launched a new version of the Saphyr Chip with three independent flowcells. When running on the two-chip Saphyr platform models that ship in 2019, the 3 X 1300 Saphyr Chip allows customers to map up to 42 whole human genomes per week or collect 1,300 Gbp (400x) coverage in less than 48 hours for samples in each of three flowcells.
The company also released a new kit for DNA isolation from blood and cells called Bionano Prep SP (solution phase). According to the company this kit dramatically reduces the time and effort needed to obtain extremely long, ultra-high molecular weight DNA by eliminating steps like plug-lysis and drop dialysis. Bionano Prep S enables users to manually process up to six patient samples in less than three hours or up to 12 patient samples per day, which reflects a savings of a full day from the workflow compared to the current plug-lysis approach, Bionano said. The new kit also costs less on a per-sample basis, the protocol is automatable, and the high purity of resulting DNA enables faster run times on the instrument and higher data quality, the company noted.
Bionano also announced the upcoming release of the latest version of Bionano Solve, its suite of data-analysis tools, which for the first time will be available in a cloud-based implementation called BioNano Compute On-Demand. The pipeline analyzes single molecules for structural variation detection at low-allele fractions, and is particularly suited for analysis of complex, heterogeneous cancer samples and samples with germline mosaicism, the company said. All major types of structural variation can be detected with more than 80 percent sensitivity when present in just 5 percent allele fraction, while translocations and inversions can be detected with more than 90 percent sensitivity at this low frequency.
Agilent Magnis NGS Prep System
Agilent has launched the Magnis NGS Prep System, a fully automated next-generation sequencing library preparation system. The firm has designed the platform to help researchers easily assay multiple genes and complex genetic aberrations from genomic DNA, including degraded samples such as formalin-fixed, paraffin-embedded specimens, Agilent said. The system comes with pre-aliquoted reagents and pre-set protocols, and is fully compatible with Agilent's SureSelect [XT HS] library prep workflow. The system can provide reproducible results with a one-day turnaround time, Agilent noted.
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