Invitrogen has launched its new Molecular Probes Qdot nanocrystal conjugated primary antibodies for use in multicolor flow cytometry. These directly labeled fluorescent reagents allow cell biologists to use an array of distinct colors to track multiple parameters within a single experiment, the company said.
When excited by a single light source, Qdot nanocrystals emit in a range of brilliant distinct colors with long-term photostability.
Invitrogen this week also launched its Click-iT glycoprotein profiling reagents. The company said that these reagents are the first commercially available glycoprotein analysis tools based on “click” chemistry.
The Click-iT method comprises two steps: an initial labeling step, in which a very small bio-orthogonal moiety is either metabolically or enzymatically incorporated into the glycan structure; and a detection step, in which a fluorescent label or biotin is selectively attached to the modified glycoprotein.
This procedure produces a very stable covalent bond that is capable of surviving mass spectrometry ionization and is compatible with multiplexed proteomics applications using 1- or 2-D gel electrophoresis or Western blot analysis to detect total proteins, total glycoproteins, or total phosphoproteins simultaneously with the Click-iT-labeled glycoproteins.
Beckman Coulter this week announced the successful integration of its Vi-CELL XR cell viability analyzer with Biomek laboratory automation workstations and other devices for real-time cellular imaging in a walk-away system for cell culture settings.
Beckman Coulter's Integrated Solutions Group developed the customized solution for a cell culture "seed-and-feed" application. Components of the solution include the Vi-CELL XR, a Biomek NXP workstation configured with a Span-8 Pod and gripper, a Biomek FXP dual-bridge workstation, storage devices, incubator, plate hotel, and two detectors.
The integrated solution provides cell analysis data necessary to evaluate clone selection, cell expansion, and protein expression results. Data from the analysis is also automatically captured for subsequent analysis with other cellular results that determine cell viability and efficiency such as confluence, loci of growth, and protein-protein interactions.