Dublin City University of Dublin, Ireland, has received US Patent No. 8,228,602, "Super critical angle fluorescence scanning system." A scanning system is described, where the system is coupled to an optical element based on super critical angle fluorescence, or SAF. According to the patent, the system is capable of fluorescence imaging of biochip arrays. The inventors claim that the use of SAF not only provides enhancement of the fluorescence collection efficiency but also confines the fluorescence detection volume to the close proximity of the biochip surface, and discriminates against fluorescence background from the analyte solution.
The Jawaharlal Nehru Center for Advanced Scientific Research and Microtest Innovations, both of Karnataka, India, have received US Patent No. 8,227,590, "High sensitivity assay for molecular typing of biological sample, probes, and a kit thereof." The patent claims a method for molecular typing of human immunodeficiency virus using surface-enhanced scattering, including resonance scattering. A kit is also claimed that consists of reagents and buffers to extract nucleic acid from the HIV; single-stranded probes to capture nucleic acid; a microarray plate to spot the capture probes; a single-stranded detector probe to detect the captured nucleic acid; and a reporter.
The New Jersey Institute of Technology of Newark has received US Patent No. 8,227,258, "Delivery and sensing of metered amounts of liquid materials." A liquid delivery apparatus is provided for depositing liquid materials onto prescribed areas, such as target areas on a microarray. The liquid delivery apparatus includes a sensing and delivery pin and a pin actuator for vertically driving the pin relative to a housing. The device also includes a photo sensor, a cavity, and a plunger configured to aspire liquid material into the cavity.
Affymetrix of Santa Clara, Calif., has received US Patent No. 8,227,253, "Oxide layers on silicon substrates for effective confocal laser microscopy." A method is described for synthesizing a polymer array. It includes: coating a substrate with an oxide layer, where the oxide layer has a thickness of at least 3,500 angstroms, and where the oxide layer is substantially transparent; derivatizing the substrate, where derivatization forms protected functional groups on the substrate, and where a functional group is protected by a labile protecting group; deprotecting the protected functional groups to remove the labile protecting group to produce an unprotected functional group; coupling each of the unprotected functional groups with a monomer, where the monomer consists of a protected functional group; and repeating the steps of deprotecting and coupling to produce the polymer array.