• siRNA duplexes should be composed of 21-nucleotide sense and antisense strands, paired to have a 2-nt 3’ overhang (a TT overhang tends to work well).
  • A particularly effective target motif is NAR(N17)YNN, where N is any nucleotide, R is an A or a G, and Y is a C or a U.
  • Target region should be selected from a cDNA sequence beginning 50 to 100 nucleotides downstream of the start codon.
  • Target cDNA sequence should have around 50 percent GC content.
  • Avoid more than three consecutive identical nucleotides.

Get the full story with
GenomeWeb Premium

Only $95 for the
first 90 days*

A trial upgrade to GenomeWeb Premium gives you full site access, interest-based email alerts, access to archives, and more. Never miss another important industry story.

Try GenomeWeb Premium now.

Already a GenomeWeb Premium member? Login Now.
Or, See if your institution qualifies for premium access.

*Before your trial expires, we’ll put together a custom quote with your long-term premium options.

Not ready for premium?

Register for Free Content
You can still register for access to our free content.

360Dx reports that the US Centers for Medicare & Medicaid Services would cover next-generation sequencing-based cancer panel tests.

The Washington Post reports that a meteorologist is being considered as presidential science and technology advisor.

In PNAS this week: precision medicine strategy to screen for disease risk, genome evolution in Haemophilus influenzae, and more.

Researchers have developed a PCR-based assay to gauge whether manatees are present in waters.