PHILDELPHIA--Scientists at the Children's Hospital of Philadelphia and researchers from collaborating institutions said they have developed a new method for identifying disease gene regions that they successfully used to map the segment of DNA known to be responsible for congenital hyperinsulinism. Direct identical by descent mapping--a quicker alternative to positional cloning--combines genomic mismatch scanning with DNA microarray technology, scientists said. To identify regions of identity, DNA from two affected subjects is split into single strands and then mixed together again. Strands with matching segments of DNA are placed on a microarray.
Hospital Researchers Develop Rapid Gene Mapping Technique
Dec 21, 1998